Wnt-11 Antibody - BSA Free

Western Blot: Wnt-11 Antibody [NBP1-31406] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Wnt-11 Antibody [NBP1-31406] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: WNT11 protein stained by WNT11 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: Wnt-11 Antibody [NBP1-31406] - Paraffin-embedded mouse colon. WNT11 antibody dilution: 1:500.

Western Blot: Wnt-11 Antibody [NBP1-31406] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Wnt-11 Antibody [NBP1-31406] - Analysis of HeLa, using NBP1-31406 at 1:200 dilution.

Immunohistochemistry-Paraffin: Wnt-11 Antibody [NBP1-31406] - Paraffin-embedded lung SCC xenograft, using antibody at 1:100 dilution.

Western Blot: Wnt-11 Antibody [NBP1-31406] -Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Wnt-11 Antibody [NBP1-31406] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt-11 antibody (NBP1-31406) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Wnt-11 Antibody [NBP1-31406] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt-11 antibody (NBP1-31406) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt-11 antibody (NBP1-31406) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, Rb
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Wnt-11 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Wnt-11. The exact sequence is proprietary.
• Localization: Secreted, extracellular space, extracellular matrix
• Predicted Species: Bovine (98%), Rabbit (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WNT11
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (85%), Xenopus laevis (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Wnt-11 Antibody - BSA Free

• HWNT11
• MGC141946
• MGC141948
• protein Wnt-11
• wingless-type MMTV integration site family, member 11
• Wnt11
• Wnt-11

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 97%, 85%, and 63% amino acid identity with mouse, chicken, and Xenopus Wnt11 protein, respectively. This gene may play roles in the development of skeleton, kidney and lung, and is considered to be a plausible candidate gene for High Bone Mass Syndrome. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Wnt-11 Antibody (NBP1-31406)(1)

Publication using NBP1-31406

• Liu T, Gonzalez De Los Santos F, Hirsch M et al. Non-canonical Wnt Signaling Promotes Myofibroblast Differentiation in Pulmonary Fibrosis American journal of respiratory cell and molecular biology 2021-06-09 [PMID: 34107237]

Bioinformatics

• Gene Symbol: WNT11
• Entrez:
  • 7481(Human)
• Uniprot:
  • O96014(Human)