Wnt-10b Antibody (904902R) [Unconjugated]

• Reactivity: Hu
• Applications: ELISA
• Clone: 904902R
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Wnt-10b Antibody (904902R) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Wnt-10b
  Met1-Lys389
  Accession # O00744
• Specificity: Detects human Wnt-10b in direct ELISAs
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Wnt-10b Antibody (904902R) [Unconjugated]

• protein Wnt-10b
• Protein Wnt-12
• SHFM6WNT-10B protein
• wingless-type MMTV integration site family, member 10B
• Wnt10b
• Wnt-10b
• WNT12
• Wnt-12

Background

Wnt-10b (also known as Wnt-12) is a 42‑44 kDa member of the Wnt family of secreted, highly conserved, cysteine-rich glycoproteins that play important roles in vertebrate pattern formation, cell fate decision, axon guidance, and tumor formation (1‑3). Human Wnt-10b cDNA encodes a 389 amino acid (aa) precursor that contains a 28 aa signal sequence plus a 361 aa mature protein that contains two glycosylation sites, three potential phosphorylation sites, and a potential palmitoylation site (3, 4). Human Wnt-10b shares 97‑99% aa identity with mouse, rat, equine, porcine, and canine Wnt-10b. Wnt-10b plays a critical role in maintaining mesenchymal stem cells and determining whether they differentiate to adipocytes or osteoblasts (5‑7). Mouse Wnt-10b deletion produces age‑dependent loss of bone mass due to defective production of osteoblasts, while transgenic over‑expression increases postnatal osteoblast differentiation and inhibits adipocyte differentiation (5‑7). Ectopic expression of Wnt-10b in an obesity and diabetes‑prone background, such as the ob/ob mouse, inhibits obesity (8). In mouse skeletal muscle, Wnt-10b is expressed inversely with SREBP1c and increases insulin sensitivity (9). In humans, a missense polymorphism is responsible for a malformation of hands and feet, while a C256Y inactivating mutation is associated with severe early-onset obesity (10, 11). Wnt-10b is mainly produced by stem cells and pre-osteoblasts, but also by adult bone marrow CD8⁺ T lymphocytes stimulated with parathyroid hormone (12). In some hepatocellular carcinomas, Wnt-10b can inhibit cancer cell growth, but in others, it can act synergistically with FGFs to stimulate cell growth (13). Several Wnts, including Wnt-10b, are expressed in both normal and/or malignant colon tissues (14).

1. Hardiman, G. et al. (1997) Cytogenet. Cell Genet. 77:278.
2. Katoh, M. (2008) Curr. Drug Targets 9:565.
3. Hausmann, G. et al. (2007) Nat. Rev. Mol. Cell Biol. 8:331.
4. Swiss Prot Accession # O00744.
5. Stevens, J.R. et al. (2010) J. Bone Miner. Res. 25:2138.
6. Bennett, C.N. et al. (2007) J. Bone Miner. Res. 22:1924.
7. Bennett, C.N. et al. (2005) Proc. Natl. Acad. Sci. USA 102:3324.
8. Wright, W.S. et al. (2007) Diabetes 56:295.
9. Abiola, M. et al. (2009) PLoS ONE 4:e8509.
10. Ugur, S.A. and A. Tolun (2008) Hum. Mol. Genet. 17:2644.
11. Christodoulides, C. et al. (2006) Diabetologia 49:678.
12. Terauchi, M. et al. (2009) Cell Metab. 10:229.
13. Yoshikawa, H. et al. (2007) Mol. Biol. Cell 18:4292.
14. Holcombe, R.F. et al. (2002) J. Clin. Pathol: Mol. Pathol. 55:220.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O00744()