WNK1 Antibody

Images

 
Western Blot: WNK1 Antibody [NB600-225] - Detection of the short form of WNK1 in F-11 cell lysate.
Immunocytochemistry/ Immunofluorescence: WNK1 Antibody [NB600-225] - WNK1 antibody was tested in HEK293 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
5 mg/ml

WNK1 Antibody Summary

Immunogen
A synthetic peptide within the C-terminal region of human WNK1 (within the last 350 amino acids). [UniProt# Q9H4A3]
Localization
Cytoplasmic
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WNK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:500 - 1:1000
Application Notes
This WNK1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In WB, this antibody recognizes the short form of WNK1, ~190 kDa. The theoretical molecular weight of human WNK1 is ~251 kDa and rat WNK1 is ~230 kDa. In ICC/IF cytomplasmic staining was observed.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
190 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
WNK1 Peptide (NB600-225PEP)
Publications
Read Publication using NB600-225.

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for WNK1 Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • Erythrocyte 65 kDa protein
  • HSAN2hereditary sensory neuropathy, type II
  • HSN2MGC163339
  • hWNK1
  • KDP
  • KDPMGC163341
  • KIAA0344
  • Kinase deficient protein
  • p65
  • PHA2C
  • PRKWNK1
  • PRKWNK1PSK
  • prostate-derived sterile 20-like kinase
  • Protein kinase lysine-deficient 1
  • Protein kinase with no lysine 1
  • protein kinase, lysine deficient 1
  • serine/threonine-protein kinase WNK1
  • WNK lysine deficient protein kinase 1
  • WNK1

Background

WNK1 (with no-lysine kinase 1) is a serine-threonine kinase regulating many ion channels implicated in sodium/potassium transport and plays a key role in regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. It is ubiquitously expressed in various tissues with higher levels in kidney and cardiovascular system, and acts as an important modulator of salt homeostasis, regulating the balance between renal sodium reabsorption and potassium excretion. WNK1 activates SCNN1A, SCNN1B, SCNN1D and SGK1, and controls sodium/chloride ion transport through WNK4 inhibition, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. Increased WNK1 expression would have the net result of increasing NaCl reabsorption, leading to volume expansion-induced hypertension. WNK1 has also been suggested to play a role in actin cytoskeletal reorganization. Defects in WNK1 are a cause of pseudohypoaldosteronism type 2C (PHA2C), and hereditary sensory and autonomic neuropathy type 2A (HSAN2A).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for WNK1 Antibody (NB600-225)(1)


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Product General Protocols

View specific protocols for WNK1 Antibody (NB600-225): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB600-225AF405
Alexa Fluor 488 Labeled NB600-225AF488
Alexa Fluor 647 Labeled NB600-225AF647
Alexa Fluor 700 Labeled NB600-225AF700
Biotin Labeled NB600-225B
DyLight 405 Labeled NB600-225V
DyLight 488 Labeled NB600-225G
DyLight 550 Labeled NB600-225R
DyLight 650 Labeled NB600-225C
FITC Labeled NB600-225F
HRP Labeled NB600-225H

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Bioinformatics

Gene Symbol WNK1
Entrez