WIZ Antibody (1110627) [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 1110627
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

WIZ Antibody (1110627) [Alexa Fluor® 488] Summary

• Immunogen: Synthetic Peptide
  Accession # O95785
• Specificity: Detects a synthetic peptide specific for human WIZ around amino acid 1100 in Direct ELISA.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for WIZ Antibody (1110627) [Alexa Fluor® 488]

• widely interspaced zinc finger motifs
• widely-interspaced zinc finger motifs
• Widely-interspaced zinc finger-containing protein
• WIZ zinc finger
• WIZ
• Zinc finger protein 803
• ZNF803
• ZNF803protein Wiz

Background

Widely interspaced zinc finger motifs (WIZ) is an epigenetic regulatory protein with a molecular weight of approximately 95 kDa. This protein plays a critical role in chromatin remodeling and transcriptional regulation by interacting with key epigenetic modifiers such as G9a/GLP and other chromatin-associated complexes. WIZ is ubiquitously expressed across various tissues and is essential for maintaining proper gene expression patterns during development and differentiation. It has been implicated in processes such as neuronal development, where it facilitates neuron-specific gene activation and repression, and in safeguarding chromatin architecture. Dysregulation of WIZ expression or function contributes to pathological conditions, including various neuropsychiatric disorders and cancer, where its role in epigenetic silencing can influence tumorigenesis and disease progression. Mutations in the WIZ gene are associated with cognitive impairments and developmental delay syndromes, suggesting its importance in neuroplasticity and brain development. As a key mediator of chromatin dynamics, WIZ serves as a promising target for research into epigenetic therapies and biomarkers of disease.

1. Mozzetta C, Pontis J, Ait-Si-Ali S. Functional Crosstalk Between Lysine Methyltransferases on Histone Substrates: The Case of G9A/GLP and Polycomb Repressive Complex 2. Antioxid Redox Signal. 2015 Jun 1;22(16):1365-81. doi: 10.1089/ars.2014.6116. Epub 2014 Dec 19. PMID: 25365549; PMCID: PMC4432786.
2. Justice M, Carico ZM, Stefan HC, Dowen JM. A WIZ/Cohesin/CTCF Complex Anchors DNA Loops to Define Gene Expression and Cell Identity. Cell Rep. 2020 Apr 14;31(2):107503. doi: 10.1016/j.celrep.2020.03.067. PMID: 32294452; PMCID: PMC7212317.
3. Simon JM, Parker JS, Liu F, Rothbart SB, Ait-Si-Ali S, Strahl BD, Jin J, Davis IJ, Mosley AL, Pattenden SG. A Role for Widely Interspaced Zinc Finger (WIZ) in Retention of the G9a Methyltransferase on Chromatin. J Biol Chem. 2015 Oct 23;290(43):26088-102. doi: 10.1074/jbc.M115.654459. Epub 2015 Sep 3. PMID: 26338712; PMCID: PMC4646261.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O95785(Mouse)