WHSC2 Antibody

Western Blot: WHSC2 Antibody [H00007469-B03P] - Analysis of WHSC2 expression in Raw 264.7.

Immunocytochemistry/Immunofluorescence: WHSC2 Antibody [H00007469-B03P] - Analysis of purified antibody to WHSC2 on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: WHSC2 Antibody [H00007469-B03P] - Analysis of WHSC2 expression in Hela S3 NE.

Western Blot: WHSC2 Antibody [H00007469-B03P] - Analysis of WHSC2 expression in NIH/3T3.

Western Blot: WHSC2 Antibody [H00007469-B03P] - Analysis of WHSC2 expression in transfected 293T cell line. Lane 1: WHSC2 transfected lysate (58.08 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

WHSC2 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: WHSC2 (NP_005654.2, 1 a.a. - 528 a.a.) full-length human protein. MASMRESDTGLWLHNKLGATDELWAPPSIASLLTAAVIDNIRLCFHGLSSAVKLKLLLGTLHLPRRTVDEMKGALMEIIQLASLDSDPWVLMVADILKSFPDTGSLNLELEEQNPNVQDILGELREKVGECEASAMLPLECQYLNKNALTTLAGPLTPPVKHFQLKRKPKSATLRAELLQKSTETAQQLKRSAGVPFHAKGRGLLRKMDTTTPLKGIPKQAPFRSPTAPSVFSPTGNRTPIPPSRTLLRKERGVKLLDISELDMVGAGREAKRRRKTLDAEVVEKPAKEETVVENATPDYAAGLVSTQKLGSLNNEPALPSTSYLPSTPSVVPASSYIPSSETPPAPSSREASRPPEEPSAPSPTLPAQFKQRAPMYNSGLSPATPTPAAPTSPLTPTTPPAVAPTTQTPPVAMVAPQTQAPAQQQPKKNLSLTREQMFAAQEMFKTANKVTRPEKALILGFMAGSRENPCQEQGDVIQIKLSEHTEDLPKADGQGSTTMLVDTVFEMNYATGQWTRFKKYKPMTNVS
• Specificity: WHSC2 - Wolf-Hirschhorn syndrome candidate 2,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: NELFA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and transfected lysate for WB. It has been used for ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for WHSC2 Antibody

• FLJ10442
• negative elongation factor A
• NELFA
• NELF-AFLJ25112
• Wolf-Hirschhorn syndrome candidate 2 protein
• Wolf-Hirschhorn syndrome candidate 2

Background

This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NELFA
• Entrez:
  • 7469(Human)
• Uniprot:
  • NP_005654.2(Human)