WHSC1 Antibody - BSA Free

Immunohistochemistry-Paraffin: WHSC1 Antibody [NBP1-30910] - Paraffin-embedded A549 xenograft, using antibody at 1:100 dilution.

Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with WHSC1 antibody [N1], N-term (NBP1-30910) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

WHSC1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit WHSC1 Antibody - BSA Free (NBP1-30910) is a polyclonal antibody validated for use in IHC and WB. Anti-WHSC1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human WHSC1. The exact sequence is proprietary.
• Localization: Nucleus, Isoform 4: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NSD2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 152 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for WHSC1 Antibody - BSA Free

• EC 2.1.1.43
• FLJ23286
• IL5 promoter REII region-binding protein
• KIAA1090
• MGC176638
• MMSETNSD2Multiple myeloma SET domain-containing protein
• multiple myeloma SET domain containing protein type III
• nuclear receptor binding SET domain protein 2
• Nuclear SET domain-containing protein 2
• probable histone-lysine N-methyltransferase NSD2
• Protein trithorax-5
• REIIBP
• trithorax/ash1-related protein 5
• TRX5
• WHS
• Wolf-Hirschhorn syndrome candidate 1 protein
• Wolf-Hirschhorn syndrome candidate 1

Background

This gene encodes a protein that contains four domains present in other developmental proteins: a PWWP domain, an HMG box, a SET domain, and a PHD-type zinc finger. It is expressed ubiquitously in early development. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene maps to the 165 kb WHS critical region and has also been involved in the chromosomal translocation t(4;14)(p16.3;q32.3) in multiple myelomas. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. Some transcript variants are nonsense-mediated mRNA (NMD) decay candidates, hence not represented as reference sequences. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for WHSC1 Antibody (NBP1-30910)(1)

Publication using NBP1-30910

• Stubbs MC, Burn TC, Sparks RB et al. The Novel Bromodomain and Extraterminal Domain Inhibitor INCB054329 Induces Vulnerabilities in Myeloma Cells That Inform Rational Combination Strategies. Clin. Cancer Res. 2018-09-11 [PMID: 30206163] (WB, Human)

Bioinformatics

• Gene Symbol: NSD2
• Entrez:
  • 7468(Human)
• Uniprot:
  • O96028(Human)