WFDC1 Antibody

Western Blot: WFDC1 Antibody [NBP2-27546] - (0.3 ug/ml) staining of Mouse Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

WFDC1 Antibody Summary

• Description: Novus Biologicals Goat WFDC1 Antibody (NBP2-27546) is a polyclonal antibody validated for use in WB and ELISA. Anti-WFDC1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RQRLHKEYPEGDSKN corresponding to internal region (near N-Terminus) according to NP_075884.1.
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: WFDC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 19 kDa band observed in mouse heart lysates (calculated MW of 23.1 kDa band according to NP_075884.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for WFDC1 Antibody

• ps20 growth inhibitor
• PS20Prostate stromal protein ps20
• WAP four-disulfide core domain 1 homolog
• WAP four-disulfide core domain 1
• WAP four-disulfide core domain protein 1

Background

WFDC1 encodes a member of the WAP-type four disulfide core domain family. The WAP-type four-disulfide core domain, or WAP signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. The encoded protein shares 81% amino acid identity with the rat ps20 protein, which was originally identified as a secreted growth inhibitor. This gene is mapped to chromosome 16q24, an area of frequent loss of heterozygosity in cancers, including prostate, breast and hepatocellular cancers and Wilms' tumor. Owing to its location and a possible growth inhibitory property of its gene product, this gene is suggested to be a tumor suppressor gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for WFDC1 Antibody (NBP2-27546)(1)

Publication using NBP2-27546

• Watson JE, Kamkar S, James K et al. Molecular analysis of WFDC1/ps20 gene in prostate cancer. Prostate 2004-10-01 [PMID: 15305342]

Bioinformatics

• Gene Symbol: WFDC1
• Entrez:
  • 67866(Mouse)
  • 171112(Rat)
• Uniprot:
  • NP_075884.1(Human)