WDTC1 Overexpression Lysate (Native)

Images

 
Western Blot: WDTC1 Overexpression Lysate (Adult Normal) [NBL1-17839] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for WDTC1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

WDTC1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC207453 and based on accession number NM_015023. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens WD and tetratricopeptide repeats 1 (WDTC1), mRNA.
Gene
WDTC1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
75.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for WDTC1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for WDTC1 Overexpression Lysate (Native)

  • adipose homolog
  • ADP
  • DCAF9
  • KIAA1037DDB1 and CUL4 associated factor 9
  • WD and tetratricopeptide repeats 1
  • WD and tetratricopeptide repeats protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-1669
Species: Hu
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P, IP
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NBP2-22106
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-31302
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-89150
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
H00011026-M01
Species: Hu
Applications: WB, ELISA
AF3499
Species: Hu
Applications: WB
H00005706-M02
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, RNAi
NBP1-82614
Species: Hu
Applications: IHC, IHC-P
AF-241-NA
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
NBP1-87040
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBL1-17839
Species: Hu
Applications: WB

Publications for WDTC1 Lysate (NBL1-17839) (0)

There are no publications for WDTC1 Lysate (NBL1-17839).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for WDTC1 Lysate (NBL1-17839) (0)

There are no reviews for WDTC1 Lysate (NBL1-17839). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for WDTC1 Lysate (NBL1-17839) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional WDTC1 Products

Bioinformatics Tool for WDTC1 Lysate (NBL1-17839)

Discover related pathways, diseases and genes to WDTC1 Lysate (NBL1-17839). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for WDTC1 Lysate (NBL1-17839)

Discover more about diseases related to WDTC1 Lysate (NBL1-17839).
 

Pathways for WDTC1 Lysate (NBL1-17839)

View related products by pathway.

Blogs on WDTC1

There are no specific blogs for WDTC1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

WDTC1 Antibody
NBP1-89234

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our WDTC1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol WDTC1