WDR79 Antibody

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Western Blot: WDR79 Antibody [NB100-68252] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, K-562, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WDR79 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

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Immunohistochemistry-Paraffin: WDR79 Antibody [NB100-68252] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-WDR79 used at a dilution of 1:200 (1ug/ml). Detection: DAB

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Immunoprecipitation: WDR79 Antibody [NB100-68252] - Detection of human WDR79 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-WDR79 antibody NB100-68252 (lot NB100-68252-2) used for IP at 6 ug per reaction. WDR79 was also immunoprecipitated by a previous lot of this antibody (lot NB100-68252-1). For blotting immunoprecipitated WDR79, NB100-68252 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP, ChIP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

WDR79 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human WD repeat domain 79 using the numbering given in entry NP_060551.1 (GeneID 55135).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WRAP53
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Immunocytochemistry/ Immunofluorescence 1:50 -1:250
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-10 ug/mg lysate
  • Knockout Validated
  • Western Blot 1:2000-1:10000
• Application Notes: KO Validation was reported in scientific literature (PMID: 27525486). ICC/IF reactivity reported in (PMID: 27525486), Chip reactivity reported in (PMID: 22547674). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Control:

  WDR44 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for WDR79 Antibody

• FLJ10385
• TCAB1WDR79telomerase Cajal body protein 1
• WD repeat containing, antisense to TP53
• WD repeat domain 79
• WD repeat-containing protein 79
• WD40 protein Wrap53
• WD40 repeat-containing protein encoding RNA antisense to p53
• WD-encoding RNA antisense to p53

Background

WD-repeat domain 79 (WDR79) contains six WD (tryptophan-aspartate) repeat domains found in a number of proteins that function as adaptor molecules in signal transduction and cytoskeletal organization. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation. The function of the WDR79 protein has not been characterized, however significant and consistent single nucleotide polymorphisms in the WDR79 gene have been found to be associated with ER negative breast cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for WDR79 Antibody (NB100-68252)(10)

Publications using NB100-68252

• Jian Qin, Alexandre Garus, Chantal Autexier The C-terminal extension of dyskerin is a dyskeratosis congenita mutational hotspot that modulates interaction with telomerase RNA and subcellular localization Human Molecular Genetics 2024-02-15 [PMID: 37879098]
• Klump BM, Perez GI, Patrick EM et al. TCAB1 prevents nucleolar accumulation of the telomerase RNA to facilitate telomerase assembly Cell reports 2023-06-01 [PMID: 37267110]
• Xu G, Yamamoto N, Nojima T, et al. HnRNP F/H associate with hTERC and telomerase holoenzyme to modulate telomerase function and promote cell proliferation Cell Death Differ. 2019-12-20 [PMID: 31863069] (WB, Human)
• MacNeil, DE;Lambert-Lanteigne, P;Autexier, C; N-terminal residues of human dyskerin are required for interactions with telomerase RNA that prevent RNA degradation Nucleic Acids Res. 2019-04-01 [PMID: 30931479] (WB, Human)
• Vogan JM, Zhang X, Youmans DT et al. Minimized human telomerase maintains telomeres and resolves endogenous roles of H/ACA proteins, TCAB1, and Cajal bodies. Elife. 2016-08-15 [PMID: 27525486] (WB, ICC/IF, Human)
• Vogan JM, Collins K. Dynamics of Human Telomerase Holoenzyme Assembly and Subunit Exchange Across the CellCycle* . Cell cycle. 2015-07-13 [PMID: 26170453] (ICC/IF, WB, Human)
• Schertzer M, Jouravleva K, Perderiset M et al. Human regulator of telomere elongation helicase 1 (RTEL1) is required for the nuclear and cytoplasmic trafficking of pre-U2 RNA Nucleic Acids Res. 2015-01-27 [PMID: 25628358]
• Sun CK, Luo XB, Gou YP et al. TCAB1: a potential target for diagnosis and therapy of head and neck carcinomas. Mol. Cancer 2014-07-28 [PMID: 25070141] (WB, IHC-P, Human)
  
  Details:
  TCAB1/ WDR79 antibody used for WB (1:2000 dilution in 5% BSA) and IHC-P (Tris-EDTA antigen retrieval for 3-5 min at 100C, primary used at 1:200 dilution) in human head and neck carcinoma cell lines/xenograft and pathological specimens. Figure 1a - WB showing elevated expression of TCAB1 in head and neck cancer cell lines (CNE1, HSC-3, Cal27, ACC2) compared to human normal primary cells (PDLC, DPC); Figure 2b - WB of TCAB1 in Cal27 cells treated or not with exogenous shTCAB1 lentivirus for TCAB1 depletion; Figure 1c - TCAB1 IHC images of human nasopharyngeal carcinoma/NPC and nasopharyngitis/NPI tissues; Figure 4 - TCAB1 IHC staining of xenograft generated using shTCAB1-C and shScra cells.
• Stern JL, Zyner KG, Pickett HA et al. Telomerase Recruitment Requires both TCAB1 and Cajal Bodies Independently Mol Cell Biol 2012-07-01 [PMID: 22547674] (Chemotaxis, ICC/IF, Human)
• Mahmoudi S, Henriksson S, Farnebo L et al. WRAP53 promotes cancer cell survival and is a potential target for cancer therapy. Cell Death Dis 2011-01-01 [PMID: 21368886]

Review for WDR79 Antibody (NB100-68252) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: I Sawyer IF Human 02/26/2016

Summary

• Application: Immunofluorescence
• Sample Tested: hela
• Species: Human
• Lot: A1

Bioinformatics

• Gene Symbol: WRAP53
• Entrez:
  • 55135(Human)
• Uniprot:
  • Q9BUR4(Human)