WDR77 Antibody (8B2F7) - BSA Free

Western Blot: WDR77 Antibody (8B2F7) [NBP3-27910] - Western blot analysis of extracts of various cell lines, using WDR77 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: 8B2F7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

WDR77 Antibody (8B2F7) - BSA Free Summary

• Description: Novus Biologicals Mouse WDR77 Antibody (8B2F7) - BSA Free (NBP3-27910) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein of human WDR77
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: WDR77
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for WDR77 Antibody (8B2F7) - BSA Free

• Androgen receptor cofactor p44
• MEP-50
• MEP50Nbla10071
• methylosome protein 50
• MGC2722
• p44
• p44/Mep50
• RP11-552M11.3
• WD repeat domain 77
• WD repeat-containing protein 77

Background

WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061). This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al., 2002 [PubMed 11756452]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WDR77
• Uniprot:
  • Q9BQA1(Human)