WDR61 Antibody (OTI4C4)

Western Blot: WDR61 Antibody (4C4) [NBP2-46359] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WDR61.

Immunohistochemistry: WDR61 Antibody (4C4) [NBP2-46359] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Western Blot: WDR61 Antibody (4C4) [NBP2-46359] - Analysis of extracts (10ug) from 5 different cell lines and 4 human tissue (1: Hela; 2: HepG2; 3: K562; 4: Mcf7; 5: SVT2; 6: Liver; 7: Testis; 8: Brain; 9: Uterus)

Immunohistochemistry: WDR61 Antibody (4C4) [NBP2-46359] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: WDR61 Antibody (4C4) [NBP2-46359] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI4C4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

WDR61 Antibody (OTI4C4) Summary

• Description: Novus Biologicals Mouse WDR61 Antibody (OTI4C4) (NBP2-46359) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 92-305 of human WDR61(NP_079510) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SKIC8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 33.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for WDR61 Antibody (OTI4C4)

• Meiotic recombination REC14 protein homolog
• REC14
• recombination protein REC14
• SKI8
• WD repeat domain 61
• WD repeat-containing protein 61

Background

WDR61 is a subunit of the human PAF and SKI complexes, which function in transcriptional regulation and are involved in events downstream of RNA synthesis, such as RNA surveillance (Zhu et al., 2005 [PubMed 16024656]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SKIC8