Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric)

ELISA: Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric) [NBP3-11045] - Standard Reference Curve

ELISA: Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric) [NBP3-11045] - Linearity was determined by using low (rat kidney) and high (mouse kidney) natural samples, diluted 1/100 and mixed in various proportions.

ELISA: Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric) [NBP3-11045] - VPS35 Determination of WT-A549 vs KN-549 Lysates Graph for the VPS35 Kit. Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay.

• Reactivity: Hu, Mu, Rt
• Applications: ELISA
• Suitable Sample Type: Cell Lysate, Tissue
• Standard Curve Range: 78.125 - 5000 pg/mL
• Sensitivity: 50 pg/mL

Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 78.125 - 5000 pg/mL
• Sensitivity: 50 pg/mL
• Assay Type: Competitive ELISA
• Spike Recovery: 95.5%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: VPS35

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4C.

Alternate Names for Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric)

• DKFZp434P1672
• FLJ10752
• FLJ13588
• FLJ20388
• hVPS35
• Maternal-embryonic 3
• MEM3DKFZp434E1211
• vacuolar protein sorting 35 (yeast homolog)
• vacuolar protein sorting 35 (yeast)
• vacuolar protein sorting 35 homolog (S. cerevisiae)
• vacuolar protein sorting-associated protein 35
• Vesicle protein sorting 35

Background

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for VPS35 ELISA Kit (NBP3-11045). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: VPS35