VPS11 Antibody - BSA Free

Western Blot: VPS11 Antibody [NBP1-31054] - Sample(30 ug of whole cell lysate)A:293TB:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:500.

Immunocytochemistry/Immunofluorescence: VPS11 Antibody [NBP1-31054] - Sample: HeLa cells were fixed in -20C 100% MeOH for 5 min. Green: VPS11 protein stained by VPS11 antibody diluted at 1:500. Blue: Hoechst 33343 staining.

Immunohistochemistry-Paraffin: VPS11 Antibody [NBP1-31054] - VPS11 antibody detects VPS11 protein at cytosol on Ca922 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Ca922 xenograft. VPS11 antibody (NBP1-31054).Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min

• Reactivity: Hu, Mu, Rt, Bv, Fi, Xp
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

VPS11 Antibody - BSA Free Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human VPS11. The exact sequence is proprietary.
• Localization: Late endosome membrane, Peripheral membrane protein, Cytoplasmic side, Lysosome membrane, Peripheral membrane protein, Cytoplasmic side
• Predicted Species: Mouse (100%), Rat (100%), Xenopus (92%), Fish (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VPS11
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (85%), Japanese Medaka (100%), Xenopus Tropicalis (92%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for VPS11 Antibody - BSA Free

• hVPS11
• PEP5
• RING finger protein 108
• RNF108END1
• vacuolar protein sorting 11 (yeast homolog)
• vacuolar protein sorting 11 homolog (S. cerevisiae)
• vacuolar protein sorting protein 11
• vacuolar protein sorting-associated protein 11 homolog

Background

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps11 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: VPS11