VPS11 Antibody

Images

 
Western Blot: VPS11 Antibody [NBP1-31054] - Sample(30 ug of whole cell lysate)A:293TB:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: VPS11 Antibody [NBP1-31054] - Sample: HeLa cells were fixed in -20C 100% MeOH for 5 min. Green: VPS11 protein stained by VPS11 antibody diluted at 1:500. Blue: Hoechst 33343 ...read more
Immunohistochemistry-Paraffin: VPS11 Antibody [NBP1-31054] - VPS11 antibody detects VPS11 protein at cytosol on Ca922 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Ca922 xenograft. VPS11 antibody ...read more
Western Blot: VPS11 Antibody [NBP1-31054] - Analysis of VPS11 Antibody on: A:293T B:Hep G2 7.5% SDS PAGE diluted at 1:500

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Fi, XpSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

VPS11 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human VPS11. The exact sequence is proprietary.
Localization
Late endosome membrane, Peripheral membrane protein, Cytoplasmic side, Lysosome membrane, Peripheral membrane protein, Cytoplasmic side
Predicted Species
Mouse (100%), Rat (100%), Xenopus (92%), Fish (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VPS11
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (85%), Japanese Medaka (100%), Xenopus Tropicalis (92%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for VPS11 Antibody

  • hVPS11
  • PEP5
  • RING finger protein 108
  • RNF108END1
  • vacuolar protein sorting 11 (yeast homolog)
  • vacuolar protein sorting 11 homolog (S. cerevisiae)
  • vacuolar protein sorting protein 11
  • vacuolar protein sorting-associated protein 11 homolog

Background

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps11 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VPS11