Human Vitronectin ELISA Kit (Colorimetric) Summary
Specificity |
Recognizes both recombinant and natural Human Vitronectin. |
Standard Curve Range |
31.25-2000 pg/ml (example only; lot dependent) |
Sensitivity |
15.19 pg/ml |
Assay Type |
Sandwich ELISA |
Inter-Assay |
CV% < 2.4 % |
Intra-Assay |
CV% < 6.2 |
Spike Recovery |
96 -110% |
Sample Volume |
100 ul |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
VTN |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage is content dependent. |
Kit Components
Components
|
- 1. 96 well microplate coated with Capture Antibody
- 2. Detection Antibody conjugated to HRP
- 3. Standards
- 4. Wash Buffer Concentrate
- 5. Dilution Buffer Concentrate
- 6. Color Reagent A
- 7. Color Reagent B
- 8. Stop Solution
|
Alternate Names for Human Vitronectin ELISA Kit (Colorimetric)
Background
Vitronectin also called serum spreading factor or complement S-protein, is a member of the pexin family (1). Found in serum and in tissues, Vitronectin exist in two forms; as a single chain (75kDa) and as a clipped form composed of two chains (65kDa and 10kDa). Vitronectin promotes cell adhesion, spreading and migration through specific interactions with cellular integrins, and inhibits cytolysis by the complement C5b-9 complex (2-4). Vitronectin also interacts with several critical proteins that regulate thrombosis and fibrinolysis. Vitronectin is phosphorylated by PKA and CKII (5).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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