Vitronectin Antibody (A27) - BSA Free

Product Discontinued

Vitronectin Antibody (A27) - BSA Free Summary

• Immunogen: Lysed bovine corneal endothelial cells and extracellular matrix
• Specificity: Highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: VTN
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:80000
  • Immunoaffinity Purification
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:50
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:200
• Application Notes: ELISA: NBP1-05055 is suitable for ELISA. (1, 2, 3) WB: NBP1-05055 is suitable for immunoblotting. IHC: NBP1-05055 immunostaining of frozen PLP-fixed sections of bovine tissues. AP: The antibody can be used as an affinity purification reagent of vitronectin from bovine plasma or serum and to quantitatively deplete plasma or serum of vitronectin. It can also be used to probe vitronectin conformation.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Vitronectin Antibody (A27) - BSA Free

• Complement S-protein
• epibolin
• Serum Spreading Factor
• Serum-spreading factor
• Somatomedin B
• S-protein
• V75
• vitronectin (serum spreading factor, somatomedin B, complement S-protein)
• Vitronectin
• VN
• VNT
• VTN

Background

Vitronectin is a plasma glycoprotein that circulates in the blood. Vitronectin is circulating as a mixture of both 75 kDa and 65 kDa forms. Vitronectin is a major cell adhesive glycoprotein and is a common component of extracellular matrix and plasma. It competes effectively with other plasma proteins and is often involved in cell attachment, regulation of blood coagulation and immune responses. It has similar tissue distribution to fibronectin and also its integrin receptor recognizes fibronectin (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VTN