visinin-like 1 Antibody - BSA Free

Western Blot: visinin-like 1 Antibody [NBP2-20862] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Visinin-like 1 antibody diluted at 1:100000.

Immunohistochemistry-Frozen: visinin-like 1 Antibody [NBP2-20862] - Visinin-like 1 antibody detects Visinin-like 1 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Visinin-like 1 protein stained by Visinin-like 1 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.

Western Blot: visinin-like 1 Antibody [NBP2-20862] - Sample (20 ug of whole cell lysate) A: mouse brain 12% SDS PAGE gel, diluted at 1:20000.

Western Blot: visinin-like 1 Antibody [NBP2-20862] - Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE gel, diluted at 1:1000.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

visinin-like 1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit visinin-like 1 Antibody - BSA Free (NBP2-20862) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human visinin-like 1. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VSNL1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Western Blot 1:500-1:100000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for visinin-like 1 Antibody - BSA Free

• HLP3Hippocalcin-like protein 3
• HPCAL3
• HUVISL1
• VILIP
• VILIP-1
• VILIPVLP-1
• visinin-like 1
• visinin-like protein 1
• VISL1
• VSNL1

Background

This gene is a member of the visinin/recoverin subfamily of neuronal calcium sensor proteins. The encoded protein is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium-dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: VSNL1
• Uniprot:
  • P62760(Human)