visinin-like 1 Antibody (PSH0-08)

Western Blot: visinin-like 1 Antibody (PSH0-08) [NBP3-33089] - Western blot analysis of visinin-like 1 on different lysates with Rabbit anti-visinin-like 1 antibody (NBP3-33089) at 1/1,000 dilution. Lane 1: Human liver tissue lysate (20 ug/Lane) Lane 2: Hela cell lysate (10 ug/Lane) Lane 3: Hela cell lysate (10 ug/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33089) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: visinin-like 1 Antibody (PSH0-08) [NBP3-33089] - Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-visinin-like 1 antibody (NBP3-33089) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33089) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: PSH0-08
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

visinin-like 1 Antibody (PSH0-08) Summary

• Description: Novus Biologicals Rabbit visinin-like 1 Antibody (PSH0-08) (NBP3-33089) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human visinin-like 1 aa 1-191 / 191. (Uniprot: P62760)
• Localization: Cytosol, membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: VSNL1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000
  • Western Blot 1:1000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for visinin-like 1 Antibody (PSH0-08)

• HLP3Hippocalcin-like protein 3
• HPCAL3
• HUVISL1
• VILIP
• VILIP-1
• VILIPVLP-1
• visinin-like 1
• visinin-like protein 1
• VISL1
• VSNL1

Background

visinin-like 1 is a member of the visinin/recoverin subfamily of neuronal calcium sensor proteins. The encoded protein is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium-dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VSNL1