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VIP Antibody (2) [DyLight 350]

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Product Details

Summary
Reactivity Hu, Rt, Po, BvSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
2
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 350

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-05163AF350
Alexa Fluor 405
NBP1-05163AF405
Alexa Fluor 488
NBP1-05163AF488
Alexa Fluor 532
NBP1-05163AF532
Alexa Fluor 594
NBP1-05163AF594
Alexa Fluor 647
NBP1-05163AF647
Alexa Fluor 700
NBP1-05163AF700
Alexa Fluor 750
NBP1-05163AF750
Biotin
NBP1-05163B
DyLight 405
NBP1-05163V
DyLight 488
NBP1-05163G
DyLight 550
NBP1-05163R
DyLight 594
NBP1-05163DL594
DyLight 650
NBP1-05163C
DyLight 680
NBP1-05163FR
DyLight 755
NBP1-05163IR
FITC
NBP1-05163F
HRP
NBP1-05163H
Janelia Fluor 549
NBP1-05163JF549
Janelia Fluor 646
NBP1-05163JF646
Unconjugated
NBP1-05163
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-05163
BSA Free
NBP1-05163
BSA Free
NBP1-05163-0.025ml
100% Guarantee on every product

VIP Antibody (2) [DyLight 350] Summary

Immunogen
Vasoactive intestinal peptide
Specificity
Binds to human, bovine, porcine or rat VIP, but does not bind to PHM or PACAP
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
VIP
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for VIP Antibody (2) [DyLight 350]

  • MGC13587
  • PHM27
  • vasoactive intestinal peptide
  • VIP peptides
  • VIP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: Substance P Antibody [NB300-187] - Staining of trigeminal ganglia from P3-P5 rat pups. BMC Neuroscience 2010, 11:32doi:10.1186/1471-2202-11-32
Immunohistochemistry: Substance P Antibody [NB300-187] - Spinal Cord Dorsal Horn (Human).
Immunocytochemistry/Immunofluorescence: Substance P Antibody [NB300-187] - Detection of Substance P in rat spinal cord dorsal horn (red fluorescence). DAPI (blue) was used as counter stain.
(7 Publications)
Species: Hu, Mu, Rt
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PACAP/ADCYAP1
Antibody [Unconjugate
AF6380
Western blot shows lysates of mouse kidney tissue. PVDF Membrane was probed with 1 µg/mL of Human PACAP/ADCYAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6380) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PACAP/ADCYAP1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2
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Neuropeptide Y
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Neuropeptide Y Antibody
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Somatostatin
Antibody (7G5)
NBP2-37447
Western Blot: Somatostatin Antibody (7G5) [NBP2-37447] - Analysis using SST mAb against human SST recombinant protein. (Expected MW is 38.2 kDa)
Immunohistochemistry-Paraffin: Somatostatin Antibody (7G5) [NBP2-37447] - Analysis of lung cancer tissues using SST mouse mAb with DAB staining.
Flow Cytometry: Somatostatin Antibody (7G5) [NBP2-37447] - Analysis of HepG2 cells using SST mouse mAb (green) and negative control (red).
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(1 Publication)
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Procalcitonin
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NB120-14817
Procalcitonin Antibody (44D9)
(1 Review)
(6 Publications)
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Applications: WB, ICC/IF, IHC, S-ELISA
Proapoptotic
Caspase Adaptor Prote ...
NBP1-92287
Western Blot: Proapoptotic Caspase Adaptor Protein Antibody [NBP1-92287] - Analysis using Anti-MZB1 antibody NBP1-92287 (A) shows similar pattern to independent antibody NBP2-55874 (B).
Immunohistochemistry-Paraffin: Proapoptotic Caspase Adaptor Protein Antibody [NBP1-92287] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Proapoptotic Caspase Adaptor Protein Antibody [NBP1-92287] - Staining of human liver, lymph node, placenta and small intestine using Anti-MZB1 antibody NBP1-92287 (A) shows similar protein distribution across tissues to independent antibody NBP2-55874 (B).
Species: Hu
Applications: WB, IHC, IHC-P
Cholecystokinin
Antibody
NBP2-62664
Immunohistochemistry-Paraffin: Cholecystokinin Antibody [NBP2-62664] - Staining of human hypothalamus shows strong positivity in neuronal projections.
Immunohistochemistry-Paraffin: Cholecystokinin Antibody [NBP2-62664] - Analysis in human duodenum and skeletal muscle tissues using Anti-CCK antibody. Corresponding CCK RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Cholecystokinin Antibody [NBP2-62664] - Staining of human duodenum shows high expression.
Species: Hu
Applications: IHC, IHC-P
Secretin Antibody
NBP2-48988
Immunohistochemistry-Paraffin: Secretin Antibody [NBP2-48988] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: Secretin Antibody [NBP2-48988] - Staining of human duodenum shows high expression.
Immunohistochemistry-Paraffin: Secretin Antibody [NBP2-48988] - Staining in human duodenum and liver tissues using anti-SCT antibody. Corresponding SCT RNA-seq data are presented for the same tissues.
Species: Hu
Applications: IHC, IHC-P
TFF2 Antibody
(366508) [Unconjugate
MAB4077
TFF2 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human TFF2 Monoclonal Antibody (Catalog # MAB4077) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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EN-RAGE/S100A12
Antibody [Unconjugate
AF1052
Western blot shows lysate of human granulocytes. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EN‑RAGE/S100A12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1052) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EN‑RAGE/S100A12 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
EN‑RAGE/S100A12 was detected in immersion fixed paraffin-embedded sections of human tonsil using 15 µg/mL Goat Anti-Human EN‑RAGE/S100A12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1052) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Prolactin Antibody
[Unconjugated]
AF1445
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Gastrin-releasing
Peptide/Bombesin/Neur ...
DY7847-05
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Glucagon Antibody
(181402) [Unconjugate ...
MAB1249
Glucagon was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using 10 µg/mL Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
beta TC‑6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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(4 Publications)
Species: Hu, Mu
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Tyrosine
Hydroxylase Antibody
NB300-109
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Immunohistochemistry-Paraffin: Neurotensin Antibody [NBP2-33903] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
Immunohistochemistry-Paraffin: Neurotensin Antibody [NBP2-33903] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
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POMC Antibody
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Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
(10 Publications)
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Immunocytochemistry/Immunofluorescence: Glucose 6 phosphate isomerase Antibody (2D2) [NBP2-02541] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Glucose 6 phosphate isomerase.
Immunohistochemistry-Paraffin: Glucose 6 phosphate isomerase Antibody (2D2) [NBP2-02541] - Staining of paraffin-embedded Human prostate tissue using anti-Glucose 6 phosphate isomerasemouse monoclonal antibody.
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Transient Overexpress ...
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Lane 1: GHRH transfected lysate (12.4 KDa) Lane 2: Non-transfected lysate.
GHRH transfected lysate.
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Pancreatic
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Flow Cytometry: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunohistochemistry-Frozen: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Analysis of Pancreatic Polypeptide in mouse adult pancreas tissue. IHC-Fr image submitted by a verified customer review.
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VIP Antibody (2)
[DyLight 350]
NBP1-05163UV
VIP Antibody (2) [DyLight 350]
Species: Hu, Rt, Po, Bv
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for VIP Antibody (NBP1-05163UV) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-05163AF350
Alexa Fluor 405 NBP1-05163AF405
Alexa Fluor 488 NBP1-05163AF488
Alexa Fluor 532 NBP1-05163AF532
Alexa Fluor 594 NBP1-05163AF594
Alexa Fluor 647 NBP1-05163AF647
Alexa Fluor 700 NBP1-05163AF700
Alexa Fluor 750 NBP1-05163AF750
Biotin NBP1-05163B
DyLight 350 NBP1-05163UV
DyLight 405 NBP1-05163V
DyLight 488 NBP1-05163G
DyLight 550 NBP1-05163R
DyLight 594 NBP1-05163DL594
DyLight 650 NBP1-05163C
DyLight 680 NBP1-05163FR
DyLight 755 NBP1-05163IR
FITC NBP1-05163F
HRP NBP1-05163H
Janelia Fluor 549 NBP1-05163JF549
Janelia Fluor 646 NBP1-05163JF646
Learn about Custom Antibody Labeling

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