VIP Antibody (2) - BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: VIP Antibody (2) [NBP1-05163] - Staining in human pancreas tissue. Signal is strong in nerves of exocrine part of pancreas. Dilution: 1:100.
Immunohistochemistry-Paraffin: VIP Antibody (2) - BSA Free [NBP1-05163] - Formalin fixed paraffin-embedded human pancreas tissue. Dilution: 1:100
Immunohistochemistry-Paraffin: VIP Antibody (2) [NBP1-05163] - Staining of human appendix tissue. Dilution: 1:100
Immunohistochemistry-Paraffin: VIP Antibody (2) [NBP1-05163] - Staining of human appendix tissue. Dilution: 1:100

Product Details

Summary
Reactivity Hu, Rt, Po, BvSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

VIP Antibody (2) - BSA Free Summary

Immunogen
Vasoactive intestinal peptide adsorbed onto aluminum hydroxide gel
Specificity
Binds to human, bovine, porcine or rat VIP, but does not bind to PHM or PACAP
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
VIP
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
The dilution guideline of 1/1000 applies to VIP coated directly at 2ug/mL. For glutaraldehyde-linked VIP-ovalbumin (molar ratio 4:1) coated at 2ug/mL, the dilution is 1/32,000.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
10mM Phosphate (pH 7.4) and 0.5M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for VIP Antibody (2) - BSA Free

  • MGC13587
  • PHM27
  • vasoactive intestinal peptide
  • VIP peptides
  • VIP

Background

Vasoactive intestinal peptide (VIP) is a 28-amino-acid peptide belonging to the secretin-glucagon family of peptide hormones. Its sequence is identical in man, ox, pig and rat. VIP is widely distributed in the central and peripheral nervous systems, acting as a neurotransmitter or neuromodulator. Peripherally it is involved in vaso- and bronchodilatation, smooth muscle relaxation and stimulation of secretion. Ectopic endocrine tumors secreting VIP (VIPomas) give rise to raised plasma levels of VIP and are associated with watery diarrhea, hypokalemia and achlorhydria syndrome. VIP shows high sequence homology with peptide histidine-methionine (PHM) in man or peptide histidine-serine (PHI) in pigs or rats, present in the same precursor, and with the neuropeptide pituitary adenylate cyclase activating polypeptide (PACAP).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for VIP Antibody (NBP1-05163). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in the NBP1-05163, your anti-VIP mAb. I noticed that the mAb has been tested in a variety of applications, but I'm curious if you are aware of it being used in mice, and more importantly in flow cytometry and IF?
    • In regards to your inquiry unfortunately this antibody has only been tested in ELISA and we have not tested it in Flow cytometry or IF. Due to this antibody being of a mouse host we have not tested on mouse samples due to high background potential. Unfortunately we do not have any samples we can provide, but we do have our Innovator's Reward if you wish to test this in untested species or applications. If you would like to test this antibody in an untested species and/or application and share your results with us, then I can recommend our Innovators Reward Program.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-05163AF350
Alexa Fluor 405 NBP1-05163AF405
Alexa Fluor 488 NBP1-05163AF488
Alexa Fluor 532 NBP1-05163AF532
Alexa Fluor 594 NBP1-05163AF594
Alexa Fluor 647 NBP1-05163AF647
Alexa Fluor 700 NBP1-05163AF700
Alexa Fluor 750 NBP1-05163AF750
Biotin NBP1-05163B
DyLight 350 NBP1-05163UV
DyLight 405 NBP1-05163V
DyLight 488 NBP1-05163G
DyLight 550 NBP1-05163R
DyLight 594 NBP1-05163DL594
DyLight 650 NBP1-05163C
DyLight 680 NBP1-05163FR
DyLight 755 NBP1-05163IR
FITC NBP1-05163F
HRP NBP1-05163H
Janelia Fluor 549 NBP1-05163JF549
Janelia Fluor 646 NBP1-05163JF646

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