VGLL2 Antibody

Western Blot: VGLL2 Antibody [NBP2-24649] - analysis of VGLL2/VITO1 in human skeletal muscle lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Product Discontinued

VGLL2 Antibody Summary

• Immunogen: A portion of amino acids 50-100 of human VGLL2/VITO1 was used as the immunogen.
• Predicted Species: Rat (100%), Monkey (90%), Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VGLL2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-3 ug/ml

Reactivity Notes

The amino acid sequence used as immunogen is 85% homologous in Mouse and Canine.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for VGLL2 Antibody

• Protein VITO1
• transcription cofactor vestigial-like 2
• transcription cofactor vestigial-like protein 2
• vestigial like 2 (Drosophila)
• VGL2
• vgl-2
• VITO1vestigial-like 2

Background

VGLL2 is the master regulator in the wing development in Drosophila, which acts through a complex by interacting with scalloped, a homolog of vertebrate TEF proteins. Northern and dot blot analyses of human adult tissues detected VGLL2 expression in skeletal muscle. In situ hybridization revealed VGLL2 expression in differentiating somites and branchial arches during mouse embryogenesis. VITO1 is the clone of VGLL2. It contains a scalloped interaction domain (SID). VITO1 is more highly expressed in differentiated myotubes from the mouse C2C12 myogenic cell line than in undifferentiated cells. During differentiation in mouse C2C12 cell line, VGLL2 mRNA levels increase and VGLL2 protein translocates from the cytoplasm to the nucleus. Interference with VITO1 expression by small interfering RNA attenuated differentiation in C2C12 muscle cells. Therefore, this protein is an important component of the myogenic program.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VGLL2
• Uniprot:
  • Q8N8G2(Human)