VDAC1 Antibody (2E10) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: VDAC1 Antibody (2E10) [H00007416-M17] - Analysis of monoclonal antibody to VDAC1 on HeLa cell. Antibody concentration 10 ug/ml

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 2E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

VDAC1 Antibody (2E10) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: VDAC1 (AAH08482.1, 156 a.a. ~ 276 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NFETAKSRVTQSNFAVGYKTDEFQLHTNVNDGTEFGGSIYQKVNKKLETAVNLAWTAGNSNTRFGIAAKYQIDPDACFSAKVNNSSLIGLGYTQTLKPGIKLTLSALLDGKNVNAGGHKLG
• Marker: Mitochondria Marker
• Specificity: VDAC1 - voltage-dependent anion channel 1 (2E10)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: VDAC1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for VDAC1 Antibody (2E10) - Azide and BSA Free

• hVDAC1
• MGC111064
• Outer mitochondrial membrane protein porin 1
• Plasmalemmal porin
• Porin 31HL
• Porin 31HM
• PORIN
• PORIN-31-HL
• VDAC
• VDAC1
• VDAC-1
• voltage-dependent anion channel 1
• voltage-dependent anion-selective channel protein 1

Background

VDAC1 is an outer membrane mitochondrial protein. The VDAC proteins are thought to form aqueous channels, or pores, through which adenine nucleotides cross the outer mitochondrial membrane. VDACs have been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT), and cyclophilin D (CypD), is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis. VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VDAC1 Antibody (H00007416-M17)(1)

Publication using H00007416-M17

• Rao J, Wu Y, Fan X et al. Facilitating Mitophagy via Pink1/Parkin2 Signaling Is Essential for the Neuroprotective Effect of beta -Caryophyllene against CIR-Induced Neuronal Injury Brain sciences 2022-06-30 [PMID: 35884674]

Bioinformatics

• Gene Symbol: VDAC1
• Entrez:
  • 7416(Human)
• Uniprot:
  • AAH08482(Human)