Vasohibin Antibody (4A3)

Western Blot: Vasohibin Antibody (4A3) [H00022846-M05] - Analysis of VASH1 expression in HepG2 (Cat # L019V1).

Immunohistochemistry-Paraffin: Vasohibin Antibody (4A3) [H00022846-M05] - Analysis of monoclonal antibody to VASH1 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml

Sandwich ELISA: Vasohibin Antibody (4A3) [H00022846-M05] - Detection limit for recombinant GST tagged VASH1 is 0.03 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IHC-P, S-ELISA
• Clone: 4A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Vasohibin Antibody (4A3) Summary

• Immunogen: VASH1 (NP_055724, 3 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GGKKVAGGGSSGATPTSAAATAPSGVRRLETSEGTSAQRDEEPEEEGEEDLRDGGVPFFVNRGGLPVDEATWERMWKHVAKIHPDGEKVAQRIRGATD
• Specificity: Reacts with vasohibin 1.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: VASH1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot
• Application Notes: This antibody is reactive against cell lysate in western blot and recombinant protein in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Vasohibin Antibody (4A3)

• KIAA1036
• KIAA1036vasohibin-1
• VASH
• VASH1
• vasohibin 1
• Vasohibin

Background

"Angiogenesis inhibitor. Inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis. This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts. Does not affect the proliferation of cancer cells in vitro, but inhibits tumor growth and tumor angiogenesis. Acts in an autocrine manner. Inhibits artery neointimal formation and macrophage

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Vasohibin Antibody (H00022846-M05)(3)

Publications using H00022846-M05

• Takano M, Shimada K, Fujii T et al. Keratin 19 as a key molecule in progression of human hepatocellular carcinomas through invasion and angiogenesis. BMC Cancer 2016-11-18 [PMID: 27863477]
• Kitajima T, Toiyama Y, Tanaka K et al. Vasohibin-1 increases the malignant potential of colorectal cancer and is a biomarker of poor prognosis. Anticancer Res. 2014-10-01 [PMID: 25275025]
• Wang Q, Tian X, Zhang C, Wang Q. Upregulation of vasohibin-1 expression with angiogenesis and poor prognosis of hepatocellular carcinoma after curative surgery. Med Oncol. 2012-12-01 [PMID: 22101788] (IHC, Human)

Bioinformatics

• Gene Symbol: VASH1
• Entrez:
  • 22846(Human)
• Uniprot:
  • NP_055724()