VAP-B Antibody

Western Blot: VAP-B Antibody [21200002] - Samples: Lane 1, Marker [kDa]: 219, 111, 83, 48, 32, 26, 17 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil , Target weight [kDa]: 27, 17, 11 (splice variants) Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.

Immunohistochemistry: VAP-B Antibody [21200002] - Normal tissues generally showed strong cytoplasmic staining usually with membranous positivity. Malignant tissues displayed similar staining pattern as their normal counterparts. Image and statement courtesy of the Human Protein Atlas (HPA).

Immunohistochemistry: VAP-B Antibody [21200002] - Staining for vesicle-associated membrane protein B/C (VAPB) in human uterine corpus. Glandular cells stained strongly while cells in the endometrial stroma did not stain or stained weakly. This image is the courtesy of the Human Proteome Resource (HPR).

ELISA: VAP-B Antibody [21200002]

Product Discontinued

VAP-B Antibody Summary

• Immunogen: This antibody is specific for the C Terminus Region of the target protein.
• Specificity: This product is specific for Human VAPB.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VAPB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:5000-1:20000
• Application Notes: This antibody is useful in ELISA, Immunohistochemistry-Paraffin and Western Blot.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for VAP-B Antibody

• ALS8
• ALS8VAMP-B/VAMP-C
• VAMP (vesicle-associated membrane protein)-associated protein B and C
• VAMP-associated 33 kDa protein
• VAMP-associated protein B/C
• VAMP-B
• VAMP-C
• VAPB
• VAP-B
• VAP-B/VAP-C
• VAPC
• VAP-C
• vesicle-associated membrane protein-associated protein B/C

Background

The protein encoded by this gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VAP-B Antibody (21200002)(2)

Publications using 21200002

• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

Bioinformatics

• Gene Symbol: VAPB
• Uniprot:
  • NM_004738(Human)