Vanilloid R-like 3/TRPV3 Antibody (S15-39)

Images

 
Western Blot: Vanilloid R-like 3/TRPV3 Antibody (S15-39) [NBP2-12918] - analysis of Rat brain membrane lysate showing detection of TrpV3 protein using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39. Load: 15 ug ...read more
Immunocytochemistry/ Immunofluorescence: Vanilloid R-like 3/TRPV3 Antibody (S15-39) [NBP2-12918] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse ...read more
Immunohistochemistry: Vanilloid R-like 3/TRPV3 Antibody (S15-39) [NBP2-12918] - Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
S15-39
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Vanilloid R-like 3/TRPV3 Antibody (S15-39) Summary

Immunogen
Synthetic peptide amino acids 458-474 (cytoplasmic C-terminus) of rat TrpV3
Localization
Membrane
Specificity
Detects approx 70kDa.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TRPV3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-10 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.1-1.0 ug/ml
  • Immunohistochemistry 1:1000
  • Immunoprecipitation 1:10-1:500
Application Notes
1 ug/ml of TrpV3 Antibody was sufficient for detection of TrpV3 in 10 ug of COS-1 cell lysate transiently transfected with TrpV3 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Vanilloid R-like 3/TRPV3 Antibody (S15-39)

  • transient receptor potential cation channel subfamily V member 3
  • transient receptor potential cation channel, subfamily V, member 3
  • TRPV3
  • vanilloid receptor 3
  • Vanilloid receptor-like 3
  • vanilloid receptor-related osmotically activated channel protein
  • Vanilloid R-like 3
  • VRL3
  • VRL-3

Background

1 ug/mL was sufficient for detection of TrpV3 in 10ug of COS-1 cell lysate transiently transfected with TrpV3 by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-12918AF405
Alexa Fluor 488 Labeled NBP2-12918AF488
Alexa Fluor 647 Labeled NBP2-12918AF647
Alexa Fluor 700 Labeled NBP2-12918AF700
DyLight 350 Labeled NBP2-12918UV
DyLight 405 Labeled NBP2-12918V
DyLight 488 Labeled NBP2-12918G
DyLight 680 Labeled NBP2-12918FR
DyLight 755 Labeled NBP2-12918IR

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Bioinformatics

Gene Symbol TRPV3
Entrez