Recombinant Human VAMP3/Cellubrevin Protein

SDS-Page: Recombinant Human VAMP3/Cellubrevin Protein [NBC1-18346]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human VAMP3/Cellubrevin Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-77 of Human VAMP3/Cellubrevin
  
  

  Source: E.coli

  Amino Acid Sequence: MSTGPTAATG SNRRLQQTQN QVDEVVDIMR VNVDKVLERD QKLSELDDRA DALQAGASQF ETSAAKLKRK YWWKNCK

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: VAMP3
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 8.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 7.5), 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human VAMP3/Cellubrevin Protein

• CEBCellubrevin
• cellubrevin
• SYB3
• Synaptobrevin-3
• VAMP-3
• vesicle-associated membrane protein 3 (cellubrevin)
• vesicle-associated membrane protein 3

Background

Cellubrevin, also known as VAMP 3, is present in recycling endosomes and endosome-derived vesicles. This protein has been implicated in recycling of transferrin receptors to the plasma membrane, secretion of alpha-granules in platelets, recycling of T-cell receptors to the immunological synapses, and membrane trafficking during cell migration. Recombinant cellubrevin was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for VAMP3/Cellubrevin Recombinant Protein (NBC1-18346)(1)

Publication using NBC1-18346

• Goossens J, Cervantes González A, Dewit N et al. Evaluation of cerebrospinal fluid levels of synaptic vesicle protein, VAMP-2, across the sporadic Alzheimer's disease continuum Alzheimer's research & therapy 2023-10-28 [PMID: 37898760] (WB)

FAQs for VAMP3/Cellubrevin Recombinant Protein (NBC1-18346). (Showing 1 - 2 of 2 FAQ).

1. I have ordered NBC1-18346 from Novus. Could you recommend an Antibody for this protein which ideally also detects the rat version of it?
   • Our antibody NB300-510 will detect the rat Cellubrevin protein. For more antibodies, please search our website for Cellubrevin antibodies and limit your search using the selection tools located on the left hand side. Please feel free to contact us if you have any additional questions.
2. We purchased this protein from you guys and I need its sequence.
   • NBC1-18346: recombinant protein, aa 1-77.

Bioinformatics

• Gene Symbol: VAMP3