VAMP-2 Antibody [Alexa Fluor® 647]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

VAMP-2 Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human VAMP-2
  Met1-Lys94
  Accession # P63027
• Specificity: Detects human, mouse, and rat VAMP-2 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) VAMP-1 is observed and less than 2% cross-reactivity with rhVAMP-3 and rhVAMP-7 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VAMP-2 Antibody [Alexa Fluor® 647]

• FLJ11460
• SYB2
• SYB2vesicle-associated membrane protein 2
• synaptobrevin 2
• synaptobrevin-2
• VAMP2
• VAMP-2
• VAMP-2vesicle associated membrane protein 2
• vesicle-associated membrane protein 2 (synaptobrevin 2)

Background

VAMP-2 (vesicle associated membrane protein 2; also synaptobrevin-2) is a 13 kDa member of the synaptobrevin family of proteins. It is a type IV transmembrane (TM) protein (i.e.- a type II TM protein whose C-terminus is almost completely transmembrane) that is found in the presynaptic terminals of neurons. VAMP-2 is targeted to presynaptic vesicles following binding to synaptophysin I. Dissociation allows for synaptic vesicle fusion at the synaptic cleft with subsequent granule release. Human VAMP-2 is 116 amino acids (aa) in length. It contains one acetylation site at Ser2, a vSNARE coiled-coil homology region (aa 31‑91), and a membrane-anchor domain (aa 95‑114). Over aa 1‑94, human VAMP-2 shares 100% and 99% aa identity with canine and mouse VAMP-2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.