VAMP-2 Antibody (541405) [Unconjugated]

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Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and mouse brain tissue. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse VAMP‑2 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clone
541405
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

VAMP-2 Antibody (541405) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human VAMP‑2
Ser2-Lys94
Accession # P63027
Specificity
Detects human and mouse VAMP‑2 in Western blots. In Western blots, no cross-reactivity with recombinant human VAMP-1, -5, -7, or -8 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
VAMP2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
MAB5136 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VAMP-2 Antibody (541405) [Unconjugated]

  • FLJ11460
  • SYB2
  • SYB2vesicle-associated membrane protein 2
  • synaptobrevin 2
  • synaptobrevin-2
  • VAMP2
  • VAMP-2
  • VAMP-2vesicle associated membrane protein 2
  • vesicle-associated membrane protein 2 (synaptobrevin 2)

Background

VAMP-2 (vesicle associated membrane protein 2; also Synaptobrevin-2) is a 13 kDa member of the Synaptobrevin family of proteins. It is a type IV transmembrane (TM) protein (i.e.- a type II TM protein whose C-terminus is almost completely transmembrane) that is found in the presynaptic terminals of neurons. VAMP-2 is targeted to presynaptic vesicles following binding to Synaptophysin I. Dissociation allows for synaptic vesicle fusion at the synaptic cleft with subsequent granule release. Human VAMP-2 is 116 amino acids (aa) in length. It contains one acetylation site at Ser2, a vSNARE coiled-coil homology region (aa 31‑91), and a membrane-anchor domain (aa 95‑114). Over aa 1‑94, human VAMP-2 shares 100% and 99% aa identity with canine and mouse VAMP-2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VAMP-2 Antibody (MAB5136)(3)

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Video Protocols

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Bioinformatics

Gene Symbol VAMP2
Uniprot