VAMP-2 Antibody (541405) [Unconjugated]

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and mouse brain tissue. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse VAMP-2 Monoclonal Antibody (Catalog # MAB5136) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VAMP-2 at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clone: 541405
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

VAMP-2 Antibody (541405) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human VAMP‑2
  Ser2-Lys94
  Accession # P63027
• Specificity: Detects human and mouse VAMP‑2 in Western blots. In Western blots, no cross-reactivity with recombinant human VAMP-1, -5, -7, or -8 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: VAMP2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VAMP-2 Antibody (541405) [Unconjugated]

• FLJ11460
• SYB2
• SYB2vesicle-associated membrane protein 2
• synaptobrevin 2
• synaptobrevin-2
• VAMP2
• VAMP-2
• VAMP-2vesicle associated membrane protein 2
• vesicle-associated membrane protein 2 (synaptobrevin 2)

Background

VAMP-2 (vesicle associated membrane protein 2; also Synaptobrevin-2) is a 13 kDa member of the Synaptobrevin family of proteins. It is a type IV transmembrane (TM) protein (i.e.- a type II TM protein whose C-terminus is almost completely transmembrane) that is found in the presynaptic terminals of neurons. VAMP-2 is targeted to presynaptic vesicles following binding to Synaptophysin I. Dissociation allows for synaptic vesicle fusion at the synaptic cleft with subsequent granule release. Human VAMP-2 is 116 amino acids (aa) in length. It contains one acetylation site at Ser2, a vSNARE coiled-coil homology region (aa 31‑91), and a membrane-anchor domain (aa 95‑114). Over aa 1‑94, human VAMP-2 shares 100% and 99% aa identity with canine and mouse VAMP-2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VAMP-2 Antibody (MAB5136)(4)

Publications using MAB5136

• LJ Davis, NA Bright, JR Edgar, MDJ Parkinson, L Wartosch, J Mantell, AA Peden, JP Luzio Organelle tethering, pore formation and SNARE compensation in the late endocytic pathway Journal of Cell Science, 2021-05-27;134(10):. 2021-05-27 [PMID: 34042162] (Western Blot, Human)
• JR Knudsen, DE Steenberg, JR Hingst, LR Hodgson, C Henriquez-, Z Li, B Kiens, EA Richter, JFP Wojtaszews, P Verkade, TE Jensen Prior exercise in humans redistributes intramuscular GLUT4 and enhances insulin-stimulated sarcolemmal and endosomal GLUT4 translocation Mol Metab, 2020-04-17;0(0):100998. 2020-04-17 [PMID: 32305516] (IHC, Mouse)
• N Vashi, SB Andrabi, S Ghanwat, M Suar, D Kumar Ca(2+)-dependent Focal Exocytosis of Golgi-derived Vesicles Helps Phagocytic Uptake in Macrophages J. Biol. Chem, 2017-02-07;292(13):5144-5165. 2017-02-07 [PMID: 28174296] (ICC, Human)
• Henri-François Renard, Maria Daniela Garcia-Castillo, Valérie Chambon, Christophe Lamaze, Ludger Johannes Shiga toxin stimulates clathrin-independent endocytosis of the VAMP2, VAMP3 and VAMP8 SNARE proteins Journal of Cell Science 2015-08-01 [PMID: 26071526]

Bioinformatics

• Gene Symbol: VAMP2
• Uniprot:
  • P63027()