USP48 Antibody (3G4)

Western Blot: USP48 Antibody (3G4) [H00084196-M01] - USP48 monoclonal antibody (M01), clone 3G4 Analysis of USP48 expression in HeLa.

Immunocytochemistry/Immunofluorescence: USP48 Antibody (3G4) [H00084196-M01] - HeLa cells were incubated for 1h at 37C with USP48 Antibody, followed by incubation with Donkey anti-mouse AF 594. ICC/IF image submitted by a verified customer review.

Immunohistochemistry-Paraffin: USP48 Antibody (3G4) [H00084196-M01] - Analysis of monoclonal antibody to USP48 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.

Sandwich ELISA: USP48 Antibody (3G4) [H00084196-M01] - Detection limit for recombinant GST tagged USP48 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 3G4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

USP48 Antibody (3G4) Summary

• Immunogen: USP48 (NP_115612, 110 a.a. ~ 219 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NLELRQALYLCPSTCSDYMLGDGIQEEKDYEPQTICEHLQYLFALLQNSNRRYIDPSGFVKALGLDTGQQQDAQEFSKLFMSLLEDTLSKQKNPDVRNIVQQQFCGEYAY
• Specificity: USP48 - ubiquitin specific protease 48
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: USP48
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA. USP48 Antibody (3G4) validated for ICC/IF from a verified customer review.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for USP48 Antibody (3G4)

• Deubiquitinating enzyme 48
• DKFZp762M1713
• EC 3.1.2.15
• EC 3.4.19.12
• FLJ11328
• FLJ20103
• FLJ23054
• FLJ23277
• MGC132556
• MGC14879
• RAP1GA1
• synUSP
• ubiquitin carboxyl-terminal hydrolase 48
• ubiquitin specific peptidase 48
• ubiquitin specific protease 31
• ubiquitin specific protease 48
• ubiquitin thioesterase 48
• Ubiquitin thiolesterase 48
• Ubiquitin-specific-processing protease 48
• USP31
• USP48

Background

This gene encodes a protein containing domains that associate it with the peptidase family C19, also known as family 2 of ubiquitin carboxyl-terminal hydrolases. Family members function as deubiquitinating enzymes, recognizing and hydrolyzing the peptide bond at the C-terminal glycine of ubiquitin. Enzymes in peptidase family C19 are involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for USP48 Antibody (H00084196-M01)(1)

Publication using H00084196-M01

• Sissy B, Edward B, Mireille R et al. Variants in USP48 encoding ubiquitin hydrolase are associated with autosomal dominant non-syndromic hereditary hearing loss. Hum Mol Genet. 2021-05-31 [PMID: 34059922]

Review for USP48 Antibody (H00084196-M01) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Edward van Beelen ICC Human 01/17/2020

Summary

• Application: Immunocytochemistry
• Sample Tested: HeLa cells
• Species: Human
• Lot: JA241-3G4

Comments

• Comments: ***Novus Innovators Program - new species or application used on a primary antibody.

Bioinformatics

• Gene Symbol: USP48
• Entrez:
  • 84196(Human)
• Uniprot:
  • NP_115612()