USP30 Overexpression Lysate (Native)

Images

 
Western Blot: USP30 Overexpression Lysate (Adult Normal) [NBL1-17656] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for USP30.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

USP30 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202538 and based on accession number NM_032663. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens ubiquitin specific peptidase 30 (USP30), mRNA.
Gene
USP30

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
58.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for USP30 Overexpression Lysate (Native)

  • Deubiquitinating Enzyme 30
  • EC 3.1.2.15
  • EC 3.4.19.12
  • FLJ40511
  • MGC10702
  • ubiquitin carboxyl-terminal hydrolase 30
  • ubiquitin specific peptidase 30
  • ubiquitin specific protease 30
  • Ubiquitin Thioesterase 30
  • Ubiquitin thiolesterase 30
  • ubiquitin-specific protease 30
  • Ubiquitin-specific-processing protease 30
  • Ub-specific protease 30
  • USP30

Background

USP30, a member of the ubiquitin-specific protease family (see USP1, MIM 603478), is a novel mitochondrial deubiquitinating (DUB) enzyme (Nakamura and Hirose, 2008 [PubMed 18287522]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol USP30