USP30 Overexpression Lysate

Images

 
Western Blot: USP30 Overexpression Lysate (Adult Normal) [NBL1-17656] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for USP30.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

USP30 Overexpression Lysate Summary

Description
Transient overexpression lysate of ubiquitin specific peptidase 30 (USP30) The lysate was created in HEK293T cells, using Plasmid ID RC202538 and based on accession number NM_032663. The protein contains a C-MYC/DDK Tag.
Gene
USP30

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
58.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for USP30 Overexpression Lysate

  • Deubiquitinating Enzyme 30
  • EC 3.1.2.15
  • EC 3.4.19.12
  • FLJ40511
  • MGC10702
  • ubiquitin carboxyl-terminal hydrolase 30
  • ubiquitin specific peptidase 30
  • ubiquitin specific protease 30
  • Ubiquitin Thioesterase 30
  • Ubiquitin thiolesterase 30
  • ubiquitin-specific protease 30
  • Ubiquitin-specific-processing protease 30
  • Ub-specific protease 30
  • USP30

Background

USP30, a member of the ubiquitin-specific protease family (see USP1, MIM 603478), is a novel mitochondrial deubiquitinating (DUB) enzyme (Nakamura and Hirose, 2008 [PubMed 18287522]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-59585
Species: Hu
Applications: IB, KO, WB
294-HG/CF
Species: Hu
Applications: BA
NBP2-38464
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
H00009960-M01
Species: Hu
Applications: ELISA, ICC/IF, WB
NBP1-93456
Species: Hu
Applications: IHC, IHC-P
NB110-40693
Species: Hu
Applications: IHC, IHC-P, IP, WB
NB110-68800
Species: Hu, Mu
Applications: ICC/IF, WB
NBP1-86692
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-33600
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
AF276
Species: Hu
Applications: Block, CyTOF-ready, Flow, ICC, IHC, KO, WB
NBP2-13304
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-24589
Species: Bv, Ca, Eq, Hu, Mu, Pm
Applications: IB, IHC, IHC-P, KO, WB
D6050
Species: Hu
Applications: ELISA
H00055031-M01
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IP, S-ELISA, WB
NBP2-31361
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-97644
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB (-), WB
NB100-1800
Species: Hu, Mu, Rt
Applications: ChIP, CHIP-SEQ, ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
NBL1-17656
Species: Hu
Applications: WB

Publications for USP30 Lysate (NBL1-17656) (0)

There are no publications for USP30 Lysate (NBL1-17656).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for USP30 Lysate (NBL1-17656) (0)

There are no reviews for USP30 Lysate (NBL1-17656). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for USP30 Lysate (NBL1-17656) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional USP30 Products

Bioinformatics Tool for USP30 Lysate (NBL1-17656)

Discover related pathways, diseases and genes to USP30 Lysate (NBL1-17656). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for USP30 Lysate (NBL1-17656)

Discover more about diseases related to USP30 Lysate (NBL1-17656).
 

Pathways for USP30 Lysate (NBL1-17656)

View related products by pathway.

PTMs for USP30 Lysate (NBL1-17656)

Learn more about PTMs related to USP30 Lysate (NBL1-17656).
 

Research Areas for USP30 Lysate (NBL1-17656)

Find related products by research area.

Blogs on USP30

There are no specific blogs for USP30, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our USP30 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol USP30