USP47 Antibody (5F9) - Azide and BSA Free

Western Blot: USP47 Antibody (5F9) [H00055031-M01] - USP47 monoclonal antibody (M01), clone 5F9 Analysis of USP47 expression in HeLa.

Immunocytochemistry/Immunofluorescence: USP47 Antibody (5F9) [H00055031-M01] - Analysis of monoclonal antibody to USP47 on HeLa cell. Antibody concentration 10 ug/ml.

Sandwich ELISA: USP47 Antibody (5F9) [H00055031-M01] - Detection limit for recombinant GST tagged USP47 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IP
• Clone: 5F9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

USP47 Antibody (5F9) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse USP47 Antibody (5F9) - Azide and BSA Free (H00055031-M01) is a monoclonal antibody validated for use in WB, ELISA, ICC/IF and IP. Anti-USP47 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: USP47 (NP_060414, 203 a.a. ~ 301 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TEQADLINELYQGKLKDYVRCLECGYEGWRIDTYLDIPLVIRPYGSSQAFASVEEALHAFIQPEILDGPNQYFCERCKKKCDARKGLRFLHFPYLLTLQ
• Specificity: USP47 - ubiquitin specific protease 47
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: USP47
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for USP47 Antibody (5F9) - Azide and BSA Free

• Deubiquitinating enzyme 47
• DKFZp686C13257
• EC 3.4.19.12
• FLJ20727
• Trf (TATA binding protein-related factor)-proximal homolog (Drosophila)
• Trf (TATA binding protein-related factor)-proximal homolog
• TRFP
• ubiquitin carboxyl-terminal hydrolase 47
• ubiquitin specific peptidase 47
• ubiquitin specific protease 47
• ubiquitin thioesterase 47
• Ubiquitin thiolesterase 47
• Ubiquitin-specific-processing protease 47
• USP47

Background

Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific peptidase (USP47) is a UBP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for USP47 Antibody (H00055031-M01)(2)

Publications using H00055031-M01

• Yang SW, Oh KH, Park E et al. USP47 and C Terminus of Hsp70-Interacting Protein (CHIP) Antagonistically Regulate Katanin-p60-Mediated Axonal Growth. J Neurosci. 2013-07-31 [PMID: 23904609]
• Peschiaroli A, Skaar JR, Pagano M et al. The ubiquitin-specific protease USP47 is a novel beta-TRCP interactor regulating cell survival. Oncogene 29(9):1384-93. 2010-03-04 [PMID: 19966869]

Bioinformatics

• Gene Symbol: USP47
• Entrez:
  • 55031(Human)
• Uniprot:
  • NP_060414(Human)