USP28 Antibody

Images

 
Western Blot: USP28 Antibody [NBP1-82904] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82904] - Staining of human lymph node shows strong nuclear positivity in reaction center cells and lymphoid cells outside reaction center.
Western Blot: USP28 Antibody [NBP1-82904] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

USP28 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:SWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARA
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
USP28
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. IF fixation permeabilization: PFA/Triton X-100.
Control Peptide
USP28 Protein (NBP1-82904PEP)
Publications
Read Publication using NBP1-82904.

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (83%), Rat (88%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for USP28 Antibody

  • Deubiquitinating enzyme 28
  • EC 3.1.2.15
  • EC 3.4.19.12
  • KIAA1515ubiquitin carboxyl-terminal hydrolase 28
  • ubiquitin specific peptidase 28
  • ubiquitin specific protease 28
  • ubiquitin thioesterase 28
  • Ubiquitin thiolesterase 28
  • Ubiquitin-specific-processing protease 28
  • USP28

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for USP28 Antibody (NBP1-82904)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol USP28
Entrez