USP13 Antibody

Western Blot: USP13 Antibody [NBP2-76972] - Western blot analysis of USP13 on different lysates using anti-USP13 antibody at 1/1,000 dilution.Positive control:Lane1: 293T Lane2: DaudiLane3: Mouse testis Lane4: Rat brain

Immunocytochemistry/Immunofluorescence: USP13 Antibody [NBP2-76972] - ICC staining USP13 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Flow Cytometry: USP13 Antibody [NBP2-76972] - Flow cytometric analysis of A431 cells with USP13 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: USP13 Antibody [NBP2-76972] - ICC staining USP13 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

USP13 Antibody Summary

• Immunogen: Recombinant protein within Human USP13 aa 570-776 / 863. (SwissProt: Q92995 Human; SwissProt: Q5BKP2 Mouse)
• Localization: Nucleus. Cytosol.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP13
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 97 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for USP13 Antibody

• Deubiquitinating enzyme 13
• EC 3.1.2.15
• EC 3.4.19.12
• Isopeptidase T-3
• IsoT-3
• ISOT3IsoT-3
• ubiquitin carboxyl-terminal hydrolase 13
• ubiquitin specific peptidase 13 (isopeptidase T-3)
• ubiquitin specific protease 13 (isopeptidase T-3)
• Ubiquitin Thioesterase 13
• Ubiquitin thiolesterase 13
• Ubiquitin-specific-processing protease 13
• USP13

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: USP13