USP10 Antibody (JU32-62)

Western Blot: USP10 Antibody (JU32-62) [NBP2-75698] - Analysis of USP10 on SiHa (1) and A549 (2) cell lysate using anti-USP10 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: USP10 Antibody (JU32-62) [NBP2-75698] - Staining USP10 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: USP10 Antibody (JU32-62) [NBP2-75698] - Analysis of paraffin-embedded human placenta tissue using anti-USP10 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: USP10 Antibody (JU32-62) [NBP2-75698] - Staining USP10 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: USP10 Antibody (JU32-62) [NBP2-75698] - Staining USP10 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: USP10 Antibody (JU32-62) [NBP2-75698] - Analysis of paraffin-embedded human liver tissue using anti-USP10 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: USP10 Antibody (JU32-62) [NBP2-75698] - Analysis of paraffin-embedded human thyroid grand tissue using anti-USP10 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: USP10 Antibody (JU32-62) [NBP2-75698] - Analysis of paraffin-embedded human colon cancer tissue using anti-USP10 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: USP10 Antibody (JU32-62) [NBP2-75698] - Analysis of paraffin-embedded human spleen tissue using anti-USP10 antibody. Counter stained with hematoxylin.

Product Discontinued

USP10 Antibody (JU32-62) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human USP10 aa 28-77 / 798. (SwissProt: Q14694 Human)
• Localization: Cytoplasm. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: USP10
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for USP10 Antibody (JU32-62)

• Deubiquitinating enzyme 10
• EC 3.4.19.12
• KIAA0190MGC2621
• ubiquitin carboxyl-terminal hydrolase 10
• ubiquitin specific peptidase 10
• ubiquitin specific protease 10
• ubiquitin thioesterase 10
• Ubiquitin Thiolesterase 10
• Ubiquitin-specific-processing protease 10
• UBPO
• Uchrp
• USP10

Background

Ubiquitin is a highly conserved protein that is covalently linked to other proteins to regulate their function and degradation. This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates. The protein is found in the nucleus and cytoplasm. It functions as a co-factor of the DNA-bound androgen receptor complex, and is inhibited by a protein in the Ras-GTPase pathway. The human genome contains several pseudogenes similar to this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: USP10