Recombinant Human UCH-L3 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

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Recombinant Human UCH-L3 Protein, CF Summary

Details of Functionality
Recombinant Human UCH-L3 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UCH-L3 concentration of 0.05-5 nM. Pre-incubation for 15 minutes with 10 mM DTT is recommended to achieve maximum activity.
Source
E. coli-derived human UCH-L3 protein
Accession #
Protein/Peptide Type
Recombinant Enzymes
Gene
UCHL3
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
E-325 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 1 mM TCEP
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human UCH-L3 Protein, CF

  • EC 3.4.19.12
  • ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
  • ubiquitin carboxyl-terminal hydrolase isozyme L3
  • Ubiquitin Thioesterase L3
  • ubiquitin thiolesterase
  • UCHL3
  • UCH-L3

Background

Ubiquitin Carboxyl-terminal Esterase L3 (UCH-L3) is a member of the peptidase C12 family of deubiquitinating enzymes. It is widely expressed with the highest levels being observed in heart, testis, thymus and striated muscle (1,2). UCH-L3 is 230 amino acids (aa) in length with a predicted molecular weight of 26.2 kDa (3). It is composed of a single N-terminal UCH domain with a short active-site crossover loop allowing UCH-L3 to process small Ubiquitin derivatives (4,5). Human UCH-L3 shares 98% aa sequence identity with the mouse and rat orthologs. UCH-L3 releases monomeric Ubiquitin from Ubiquitin-protein conjugates (4). Additionally, it cleaves a GGLRQ peptide from the C-terminus of the Ubiquitin-like protein NEDD8, allowing NEDD8 to be conjugated to target proteins (2). UCH-L3 activity is muted in the presence of Ubiquitin dimers (6). UCH-L3 is suggested to function in the central nervous system. In particular, it is involved in the maintenance of neurons of the gracile tract, nucleus tractus solitaris, and area postrema, and in working memory (7,8). UCH-L3 has also been shown to modulate germ cell apoptosis and promote insulin signaling and adipogenesis (9,10). Additionally, UCH-L3 expression has been shown to be correlated with cancer (11-13).

  1. Kurihara, L.J. et al. (2000) Mol. Cell. Biol. 20:2498.
  2. Wada, H. et al. (1998) Biochem. Biophys. Res. Commun. 251:688.
  3. Wilkinson, K.D. et al. (1989) Science 246:670.
  4. Larsen, C.N. et al. (1998) Biochemistry 37:3358.
  5. Zhou, Z.R. et al. (2012) Biochem. J. 441:143.
  6. Setsuie, R. et al. (2009) Neurochem. Int. 54:314.
  7. Kurihara, L.J. et al. (2001) Hum. Mol. Genet. 10:1963.
  8. Wood, M.A. et al. (2005) Hippocampus 15:610.
  9. Kwon, J. (2007) Exp. Anim. 56:71.
  10. Suzuki, M. et al. (2009) Endocrinology 150:5230.
  11. Nam, M.J. et al. (2003) Proteomics 3:2108.
  12. Miyoshi, Y. et al. (2006) Cancer Sci. 97:523.
  13. Fang, Y. et al. (2012) Mol. Cell. Biochem. 359:59.

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Publications for UCH-L3 (E-325)(3)

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Bioinformatics

Gene Symbol UCHL3
Entrez
Uniprot