Uroplakin Ia Antibody (UPK1A/2921)

Immunohistochemistry-Paraffin: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733] - Analysis of canine bladder tissue using Uroplakin Ia antibody. Uroplakin Ia antibody used at 1:500 concentration in 3% BSA and left at 4C overnight on paraffin embedded canine bladder tissue. HIER was performed in Tris/EDTA buffer, pH 9. Image from verified customer review.

Immunohistochemistry-Paraffin: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733] - Formalin-fixed, paraffin-embedded human Urothelial Carcinoma stained with Uroplakin Ia Antibody (UPK1A/2921).

Immunohistochemistry-Paraffin: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733] - Formalin-fixed, paraffin-embedded human Urinary Bladder stained with Uroplakin Ia Antibody (UPK1A/2921).

Immunohistochemistry-Paraffin: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733] - Formalin-fixed, paraffin-embedded human Urothelial Carcinoma stained with Uroplakin Ia Antibody (UPK1A/2921).

Protein Array: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Uroplakin Ia Antibody (UPK1A/2921) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

• Reactivity: Hu, Ca
• Applications: ELISA, IHC, MA
• Clone: UPK1A/2921
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Uroplakin Ia Antibody (UPK1A/2921) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79868)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant fragment (around aa 114-173) of human Uroplakin Ia (UPK1A) protein (exact sequence is proprietary) (Uniprot: O00322)
• Localization: Cell Surface
• Marker: Urothelial Differentiation Marker
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: UPK1A
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
• Application Notes: ELISA: For coating, order Ab without BSA.
  Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Canine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Uroplakin Ia Antibody (UPK1A/2921)

• TSPAN21
• UP1A
• UPIA
• UPK1A uroplakin 1A
• UPK1A
• UPKA
• Uroplakin Ia

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Uroplakin Ia Antibody (NBP2-79733) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Canine.

reviewed by: Verified Customer Affinity Purification Canine 03/08/2022

Summary

• Application: Affinity Purification
• Sample Tested: Bladder tissue
• Species: Canine
• Lot: 11045-1P210924

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

Bioinformatics

• Gene Symbol: UPK1A
• Uniprot:
  • O00322(Human)