Uromodulin Antibody (10.32) [FITC]

Immunohistochemistry-Paraffin: Uromodulin Antibody (10.32) [FITC] [NBP1-50431] - Analysis of anti-UMOD / Uromodulin antibody with human kidney at dilution 1:50.

Product Discontinued

Uromodulin Antibody (10.32) [FITC] Summary

• Immunogen: Human Uromodulin protein.
• Specificity: Anti-human Uromodulin is a monoclonal antibody which reacts with an epitope of the urinary mucoprotein. Uromodulin is a glycoprotein of approximately 80 kDa containing up to 25% carbohydrate by weight.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: UMOD
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50
• Application Notes: For IHC-Paraffin, use heat induced antigen retrieval in pH 6.0 citrate buffer.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and EIA grade BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for Uromodulin Antibody (10.32) [FITC]

• ADMCKD2
• FJHN
• HNFJ
• HNFJ1
• MCKD2
• Tamm-Horsfall glycoprotein
• Tamm-Horsfall urinary glycoprotein
• THGP
• THP
• UMOD
• uromodulin (uromucoid, Tamm-Horsfall glycoprotein)
• Uromodulin
• Uromucoid

Background

This gene encodes uromodulin, the most abundant protein in normal urine. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. Uromodulin may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of uromodulin in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the autosomal dominant renal disorders medullary cystic kidney disease-2 (MCKD2) and familial juvenile hyperuricemic nephropathy (FJHN). These disorders are characterized by juvenile onset of hyperuricemia, gout, and progressive renal failure. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same isoform. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Uromodulin Antibody (NBP1-50431)(2)

Publications using NBP1-50431

• Radtke Aj, Chu Cj, Yaniv Z Et Al. IBEX: An open and extensible method for high content multiplex imaging of diverse tissues arXiv preprint 2021-01-01
• Radtke AJ, Chu CJ, Yaniv Z Et al. IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues Nat Protoc 2022-01-13 [PMID: 35022622] (Hydrolysis Assay, Human)
  
  Details:
  Citation using the Alexa Fluor 532 version of this antibody.

Bioinformatics

• Gene Symbol: UMOD