uPAR Antibody (62022) [Unconjugated]

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." > MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." title="Human peripheral blood granulocytes were stained with Mouse Anti-Human uPAR Monoclonal Antibody (Catalog # MAB807, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." />

Human peripheral blood granulocytes were stained with Mouse Anti-Human uPAR Monoclonal Antibody (Catalog # MAB807, filled histogram) or isotype control antibody (Catalog # <a class=

Localization of CTSB and members of the proteolytic cascade in the caveolae. Representative WB showing expression of (A) CTSB (pro-CTSB, sc-CTSB, dc-CTSB) and CAV1, or (B) ANXA2, pro-uPA and uPAR in caveolae-enriched fractions (2–5, 15 μL) and cell lysates (CL) from hTM cells. l.e.: lower exposure; h.e.: higher exposure. (C) Representative WB showing expression of pro-CTSB, pro-uPA, and uPA in conditioned media (15 μL) from hTM cells. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33379277), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunoblot image are shown (A), along with densitometric analysis of (B) PDGFR beta , (C) Cyr61, (D) CD97, (E) Glypican-1, (F) MUC18/CD146 and (G) uPAR. (H) A summary of proteins detected by mass spectrometry and immunoblot. Graphs represent mean ± SEM of n = 3 independent biological experiments, shown as 1,2,3. Unpaired t-test was used as statistical test. *P < 0.05, **P < 0.01, ***P < 0.001 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36171564), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunoblot image are shown (A), along with densitometric analysis of (B) PDGFR beta , (C) Cyr61, (D) CD97, (E) Glypican-1, (F) MUC18/CD146 and (G) uPAR. (H) A summary of proteins detected by mass spectrometry and immunoblot. Graphs represent mean ± SEM of n = 3 independent biological experiments, shown as 1,2,3. Unpaired t-test was used as statistical test. *P < 0.05, **P < 0.01, ***P < 0.001 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36171564), licensed under a CC-BY license. Not internally tested by R&D Systems.

807-UK) was serially diluted and captured by Mouse Anti-Human uPAR Monoclonal Antibody (Catalog # MAB807) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF807) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" > 807-UK) was serially diluted and captured by Mouse Anti-Human uPAR Monoclonal Antibody (Catalog # MAB807) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF807) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" title="Recombinant Human uPAR (Catalog # 807-UK) was serially diluted and captured by Mouse Anti-Human uPAR Monoclonal Antibody (Catalog # MAB807) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF807) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" />

Recombinant Human uPAR (Catalog # <a class=

• Reactivity: Hu
• Applications: WB, Flow, B/N, CyTOF-ready, ELISA(Cap)
• Clone: 62022
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

uPAR Antibody (62022) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human uPAR
  Leu23-Arg303
  Accession # Q03405
• Specificity: Detects human uPAR in direct ELISAs and Western blots. When used in a sandwich ELISA in combination with the biotinylated anti-human uPAR detection antibody (Catalog # BAF807), no significant cross-reactivity was observed with recombinant mouse uPAR.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLAUR
• Purity Statement: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Human uPAR Biotinylated Antibody (Catalog number BAF807)
  Standard: Recombinant Human uPAR (Catalog number 807-UK)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for uPAR Antibody (62022) [Unconjugated]

• CD87 antigen
• CD87
• Monocyte activation antigen Mo3
• plasminogen activator, urokinase receptor
• PLAUR
• uPAR
• U-PAR
• UPARurokinase plasminogen activator surface receptor
• u-plasminogen activator receptor form 2
• URKRMO3

Background

The urokinase-type Plasminogen Activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction. uPA is synthesized and released from cells as a
single-chain (sc) pro-enzyme with limited enzymatic activity and is converted to an active two-chain (tc) disulfide-linked active enzyme by plasmin and other specific proteinases. Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity. The enzymatic activity of scuPA has also been shown to be enhanced by binding to uPAR. Independent of their proteolytic activity, the uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. uPAR cDNA encodes a 335 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, five potential N-linked glycosylation sites and a
C‑terminal GPI-anchor site. An alternate spliced variant of uPAR encoding a secreted soluble form of uPAR also exists. Human and mouse uPAR share approximately 60% aa sequence identity and the receptor-ligand interaction is strictly species-specific.

1. Dear, A.E. and R.L. Medcalf (1988) Eur. J. Biochemistry 252:185.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for uPAR Antibody (MAB807)(22)

Publications using MAB807

• N McNamee, LR de la Fuen, MJ Santos-Mar, L O'Driscoll Proteomics profiling identifies extracellular vesicles&#039; cargo associated with tumour cell induced platelet aggregation BMC Cancer, 2022-09-29;22(1):1023. 2022-09-29 [PMID: 36171564] (Western Blot, Human)
• K Mukherjee, C Gu, A Collins, M Mettlen, B Samelko, MM Altintas, YR Sudhini, X Wang, R Bouley, D Brown, BP Pedro, SL Bane, V Gupta, PT Brinkkoett, H Hagmann, J Reiser, S Sever Simultaneous stabilization of actin cytoskeleton in multiple nephron-specific cells protects the kidney from diverse injury Nature Communications, 2022-05-03;13(1):2422. 5/3/2022 [PMID: 35504916]
• S Sharma, T Watanabe, T Nishimoto, T Takihara, L Mlakar, XX Nguyen, M Sanderson, Y Su, RA Chambers, C Feghali-Bo E4 engages uPAR and enolase-1 and activates urokinase to exert antifibrotic effects JCI Insight, 2021-12-22;6(24):. 2021-12-22 [PMID: 34935642] (Immunoprecipitation, Western Blot, Human)
• Alison G. Abraham, Yunwen Xu, Jennifer L. Roem, Jason H. Greenberg, Darcy K. Weidemann, Venkata S. Sabbisetti et al. Variability in CKD Biomarker Studies: Soluble Urokinase Plasminogen Activator Receptor (suPAR) and Kidney Disease Progression in the Chronic Kidney Disease in Children (CKiD) Study Kidney Medicine 10/25/2021 [PMID: 34693253]
• Huajiao Guo, Fuhao Wang, Yuwen Diao, Zhe Zhang, Qiuyan Chen, Chao‐Nan Qian et al. Knockdown of Notch1 inhibits nasopharyngeal carcinoma cell growth and metastasis via downregulation of CCL2, CXCL16, and uPA Molecular Carcinogenesis 10/1/2019 [PMID: 31270884]
• M van Veen, E Matas-Rico, K van de Wet, D Leyton-Pui, KM Kedziora, V De Lorenzi, Y Stijf-Bult, B van den Br, K Jalink, N Sidenius, A Perrakis, WH Moolenaar Negative regulation of urokinase receptor activity by a GPI-specific phospholipase C in breast cancer cells Elife, 2017-08-29;6(0):. 2017-08-29 [PMID: 28849762] (Flow Cytometry, Human)
• FB Al-Taweel, CW Douglas, SA Whawell The periodontal pathogen Porphyromonas gingivalis preferentially interacts with oral epithelial cells in S phase of the cell cycle Infect Immun, 2016-06-23;0(0):. 2016-06-23 [PMID: 27091929] (Flow Cytometry, Human)
• Gilder A, Jones K, Hu J, Wang L, Chen C, Carter B, Gonias S Soluble Urokinase Receptor Is Released Selectively by Glioblastoma Cells That Express Epidermal Growth Factor Receptor Variant III and Promotes Tumor Cell Migration and Invasion. J Biol Chem, 2015-04-02;290(24):14798-809. 2015-04-02 [PMID: 25837250] (Neutralization, Human)
• Thiruvengadam Arumugam, Defeng Deng, Laura Bover, Huamin Wang, Craig D. Logsdon, Vijaya Ramachandran New Blocking Antibodies against Novel AGR2–C4.4A Pathway Reduce Growth and Metastasis of Pancreatic Tumors and Increase Survival in Mice Molecular Cancer Therapeutics 2015-04-01 [PMID: 25646014] (Western Blot, Human)
• Thiruvengadam Arumugam, Defeng Deng, Laura Bover, Huamin Wang, Craig D. Logsdon, Vijaya Ramachandran New Blocking Antibodies against Novel AGR2–C4.4A Pathway Reduce Growth and Metastasis of Pancreatic Tumors and Increase Survival in Mice Molecular Cancer Therapeutics 4/1/2015 [PMID: 25646014]
• Kacsinta A, Rubenstein C, Sroka I, Pawar S, Gard J, Nagle R, Cress A Intracellular modifiers of integrin alpha 6p production in aggressive prostate and breast cancer cell lines. Biochem Biophys Res Commun, 2014-10-22;454(2):335-40. 2014-10-22 [PMID: 25450398] (Western Blot, Human)
• Huang J, Liu G, Zhang Y, Cui Z, Wang F, Liu X, Chu R, Zhao M Urinary soluble urokinase receptor levels are elevated and pathogenic in patients with primary focal segmental glomerulosclerosis. BMC Med, 2014-05-20;12(0):81. 2014-05-20 [PMID: 24884842] (Neutralization, Human)
• Tersteeg C, de Maat S, De Meyer S, Smeets M, Barendrecht A, Roest M, Pasterkamp G, Fijnheer R, Vanhoorelbeke K, de Groot P, Maas C Plasmin cleavage of von Willebrand factor as an emergency bypass for ADAMTS13 deficiency in thrombotic microangiopathy. Circulation, 2014-01-21;129(12):1320-31. 2014-01-21 [PMID: 24449821] (Functional Assay, Human)
• Isis C. Sroka, Cynthia P. Sandoval, Harsharon Chopra, Jaime M. C. Gard, Sangita C. Pawar, Anne E. Cress Macrophage-Dependent Cleavage of the Laminin Receptor alpha 6 beta 1 in Prostate Cancer Molecular Cancer Research 10/1/2011 [PMID: 21824975]
• Aliakbar Taherian, Xinlei Li, Yongqing Liu, Thomas A Haas Differences in integrin expression and signaling within human breast cancer cells BMC Cancer 2011-12-01 [PMID: 21752268] (Flow Cytometry, Human)
• Aliakbar Taherian, Xinlei Li, Yongqing Liu, Thomas A Haas Differences in integrin expression and signaling within human breast cancer cells BMC Cancer 12/1/2011 [PMID: 21752268]
• Vergiliana JF, Asokananthan N, Stewart GA Activation of the plasma kallikrein-kinin system on human lung epithelial cells. Biol. Chem., 2010-09-01;391(9):1067-77. 2010-09-01 [PMID: 20536386] (Flow Cytometry, ICC, IHC-P, Human)
• Hu H, Kwun J, Aizenstein BD, Knechtle SJ Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Transplantation, 2009-06-27;87(12):1814-20. 2009-06-27 [PMID: 19543058] (Antibody Array Development, Human)
• Schnickmann S, Camacho-Trullio D, Bissinger M, Eils R, Angel P, Schirmacher P, Szabowski A, Breuhahn K AP-1-controlled hepatocyte growth factor activation promotes keratinocyte migration via CEACAM1 and urokinase plasminogen activator/urokinase plasminogen receptor. J. Invest. Dermatol., 2008-11-20;129(5):1140-8. 2008-11-20 [PMID: 19020551] (Neutralization, Western Blot, Human)
• Gonzalez RM, Seurynck-Servoss SL, Crowley SA Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008-04-19;7(6):2406-14. 2008-04-19 [PMID: 18422355] (ELISA Microarray Development, Human)
• Brooks AM, Bates ME, Vrtis RF, Jarjour NN, Bertics PJ, Sedgwick JB Urokinase-type plasminogen activator modulates airway eosinophil adhesion in asthma. Am. J. Respir. Cell Mol. Biol., 2006-05-25;35(4):503-11. 2006-05-25 [PMID: 16728704] (Neutralization, Human)
• Spijkers PP, da Costa Martins P, Westein E, Gahmberg CG, Zwaginga JJ, Lenting PJ LDL-receptor-related protein regulates beta2-integrin-mediated leukocyte adhesion. Blood, 2004-08-24;105(1):170-7. 2004-08-24 [PMID: 15328156] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: PLAUR
• Uniprot:
  • Q03405()