Anti-uPAR Antibodies: Novus Biologicals

MAB807

uPAR Antibody (62022) [Unconjugated]

100 μg

MAB807

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood granulocytes were stained with Mouse Anti-Human uPAR Monoclonal Antibody (Catalog # MAB807, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

$Localization of CTSB and members of the proteolytic cascade in the caveolae. Representative WB showing expression of (A) CTSB (pro-CTSB, sc-CTSB, dc-CTSB) and CAV1, or (B) ANXA2, pro-uPA and uPAR in caveolae-enriched fractions (2–5, 15 μL) and cell lysates (CL) from hTM cells. l.e.: lower exposure; h.e.: higher exposure. (C) Representative WB showing expression of pro-CTSB, pro-uPA, and uPA in conditioned media (15 μL) from hTM cells. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33379277), licensed under a CC-BY license. Not internally tested by R&D Systems.$

Immunoblot image are shown (A), along with densitometric analysis of (B) PDGFR beta , (C) Cyr61, (D) CD97, (E) Glypican-1, (F) MUC18/CD146 and (G) uPAR. (H) A summary of proteins detected by mass spectrometry and immunoblot. Graphs represent mean ± SEM of n = 3 independent biological experiments, shown as 1,2,3. Unpaired t-test was used as statistical test. *P < 0.05, **P < 0.01, ***P < 0.001 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36171564), licensed under a CC-BY license. Not internally tested by R&D Systems.

807-UK) was serially diluted and captured by Mouse Anti-Human uPAR Monoclonal Antibody (Catalog # MAB807) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF807) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Human uPAR (Catalog # <a class=

807-UK) was serially diluted and captured by Mouse Anti-Human uPAR Monoclonal Antibody (Catalog # MAB807) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF807) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB807U-100UG
Alexa Fluor 405	FAB807V-100UG
Alexa Fluor 488	FAB807G-100UG
Alexa Fluor 532	FAB807X
Alexa Fluor 594	FAB807T-100UG
Alexa Fluor 647	FAB807R-100UG
Alexa Fluor 700	FAB807N-100UG
Alexa Fluor 750	FAB807S-100UG
Alexa Fluor Plus 405	FAB807AFP405-100UG
Alexa Fluor Plus 488	FAB807AFP488-100UG
Alexa Fluor Plus 555	FAB807AFP555-100UG
Alexa Fluor Plus 594	FAB807AFP594-100UG
Alexa Fluor Plus 647	FAB807AFP647-100UG
Alexa Fluor Plus 680	FAB807AFP680-100UG
Allophycocyanin	FAB807A
Allophycocyanin/Cy7	FAB807APCCY7
Biotin	FAB807B
CoraFluor 1	FAB807CL1
DyLight 350	FAB807D
DyLight 405	FAB807E
DyLight 488	FAB807K
DyLight 550	FAB807L
DyLight 594	FAB807M
DyLight 650	FAB807W
DyLight 680	FAB807Y
DyLight 755	FAB807Z
FITC	FAB807F
HRP	FAB807H
Janelia Fluor 525	FAB807JF525
Janelia Fluor 549	FAB807I
Janelia Fluor 646	FAB807J
Janelia Fluor 669	FAB807JF669
PE/Atto594	FAB807PEATT594
PE/Cy5.5	FAB807PECY55
PE/Cy7	FAB807PECY7
PerCP	FAB807C
Phycoerythrin	FAB807P
mFluor Violet 450 SE	FAB807MFV450
mFluor Violet 500 SE	FAB807MFV500
mFluor Violet 610 SE	FAB807MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB807P
Carrier Free	MAB807-SP
Carrier Free	MAB807-500
Carrier Free	FAB807V-100UG
Carrier Free	FAB807U-100UG
Carrier Free	FAB807T-100UG
Carrier Free	FAB807S-100UG
Carrier Free	FAB807R-100UG
Carrier Free	FAB807N-100UG
Carrier Free	FAB807G-100UG

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AF534

uPAR Antibody [Unconjugated]

(18 Publications)

100 μg

AF534

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

uPAR was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF534) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="uPAR was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF534) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

Raw264.7 cells were stained with Goat Anti-Mouse uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF534, filled histogram) or isotype control antibody (Catalog # <a class=

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

$Expression of murine Plaur in AT84 cells.In vitro characterization of AT84 cells stably transfected with either empty vector (EV) or a vector containing cDNA encoding murine uPAR (Plaur). A: Western blot analysis of whole cell lysates using a polyclonal anti-murine uPAR antibody (AF534). A total of 7.5 ng of recombinant murine uPAR (rmuPAR) was loaded as a positive control. Re-probing for beta -actin was used as a loading control. B: Western blot analysis of cellular membrane fractions using a polyclonal anti-murine uPAR antibody (AF534). Total protein was measured per sample and 53.5 µg of protein was loaded per lane. A and B: Images were cropped, as no additional bands were detectable. C: FACS analysis of non-permeabilized cells using a polyclonal anti-murine uPAR antibody (AF534). Alexa Fluor 488 anti-goat secondary antibody (A11055) was used as the secondary antibody. The quantified mean Alexa 488 fluorescence signal per cell line is presented in the panel to the right. D and E: Relative Plaur mRNA (uPAR) (D) or Plau mRNA (uPA) (E) expression levels as analysed using RT-qPCR. All expression levels were normalized to the expression of the reference genes Trfc and beta -actin. Error bars represent the standard error of mean (+SEM) and N = 3. One-way ANOVA; *p<0.05. F: Plasminogen-gelatin (upper panel) and gelatin (lower panel) zymography analysis of conditioned medium of cells cultured for 24 hours in SFM. HMW-uPA and mPLM (mouse plasmin) were loaded as positive controls. The images were cropped to size. G: Relative Plasminogen mRNA (Plg) expression levels as analysed using RT-qPCR. Error bars represent standard error of mean (+SEM) and N = 3. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.$

<b>Biological Strategies Validation. </b>Leiomyoma conditioned medium induced uPAR expression.Analysis of uPAR expression induced by the LCM or purified ECM proteins in cultured uPAR knock-down cells. All Western blots were performed on whole cell lysates, and uPAR was detected using the polyclonal anti-murine uPAR antibody (AF534). A: Cells were either cultured in LCM (LM) or serum free medium (SF) for 24 hours. Cells were harvested with sample buffer and re-probing for beta -actin was used as a loading control. B: uPAR1-NT cells were seeded on different ECM protein substrates, incubated for 24 hours and harvested using RIPA buffer. 7.5 µg of total protein was loaded per lane. Equal loading was verified by re-probing for beta -actin. The poloxamer pluronic was used as a no-adhesion control. Col I = Collagen I, Vn = Vitronectin, Fn = Fibronectin, Lm = Laminin, ECL = Entactin, Collagen, Laminin, and FBS = Foetal Bovine Serum. C: Cells cultured in LCM (LM) or serum free medium (SF) for 24 hours were harvested using sample buffer and deglycosylated by PNGase F treatment (+) as indicated. Re-probing for beta -actin was used as a loading control. The three bands detected by the anti-uPAR antibody are labelled 1, 2, and 3, respectively. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.

Tumour microenvironment induced uPAR protein expression in tongue tumours.Tumour growth pattern and uPAR protein levels in tongue tumours generated from the EV1, EV2, uPAR1 and uPAR2 cells. A–B: Representative images depicting the tumour growth pattern at the tumour-stroma interface in hematoxylin/eosin stained EV1 (A) and uPAR1 (B) tumours. Images were recorded at 10x magnification. C–D: Representative images depicting the IHC uPAR staining of the EV1 (C) or uPAR1 tumours (D). Images were recorded at 4x magnification. E–H: The images show high power magnification (20x magnifications) of the EV1 (E), uPAR1 (F), EV2 (G) and uPAR2 (H) tumours IHC stained for uPAR protein. Positive uPAR staining is seen as brown colour, and counterstaining was done with haematoxylin. I: The average staining index (SI) of the uPAR staining in the tumours. Maximum obtainable score is 9. The error bars shows the +SEM. N = number of tumours; EV1, N = 8/10; EV2, N = 5/10; uPAR1, N = 4/10; uPAR2 N = 9/10. One-way ANOVA; **p<0.01, *p<0.05. T = Tumours, S = Stroma. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.

Leiomyoma stroma is a strong inducer of uPAR expression.Representative images of low- (EV1-sh3) and high- (uPAR1-NT) uPAR-expressing cells invading the ex vivo leiomyoma tissue. Cells were incubated for 7 and 14 days, as indicated. The tissue was IHC stained for uPAR. Positive uPAR staining is seen as brown colour, counterstained with haematoxylin. Images were recorded at 10x magnification. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>TGF-beta 1 reduces uPAR cleavage through induced PAI-1 expression. a, f-h. Western blot analysis of whole cell lysates of cultured and stimulated AT84-uPAR cells. Where indicated, cell lysates were either treated with PNGase F (+) or samples received the same treatment except that PNGase F was omitted (−). Glycosylated uPAR is indicated as uPARglc. d-e. Western blot analysis of conditioned medium from equally seeded amounts of AT84-EV and AT84-uPAR cells. a. Cells cultured in FBSM with or without 2 ng/ml active human TGF-beta 1 for 24 h. b-c. Total RNA from treated (TGF-beta 1) or untreated (Ctrl) cells was isolated and the relative expression of uPAR mRNA (B) or uPA mRNA (C) was analysed using RT-qPCR. The error bars show the +SD. N = 3. d. Cells cultured in SFM or FBSM and stimulated with 2 ng/ml TGF-beta 1 for 24 h as indicated and PAI-1 protein levels were analysed. Controls received either no additives (−) or the TGF-beta 1 buffer (0). Positive control: recombinant mouse PAI-1 (rmPAI-1). e. AT84-uPAR cells cultured in either SFM or in FBSM. Cells were either unstimulated (−) or stimulated (+) with 2 ng/ml TGF-beta 1 and/or 10 μM of the TGF-beta 1-inhibitor SB431542 as indicated. f. AT84-uPAR cells were cultured for 24 h in FBSM and stimulated with 2 ng/ml TGF-beta 1 as indicated. g. Deglycosylated whole cell lysates from AT84-uPAR cells cultured in SFM stimulated with 2 ng/ml TGF-beta 1 +/− the inhibitor SB431542 as indicated were analysed for uPAR protein levels. h. Cells were cultured for 24- or 48 h in SFM, FBSM or TMEM. Cells were treated with 2 ng/ml TGF-beta 1 in 10% FBS as indicated. The LRP1 protein is indicated. Arrowhead shows an unknown band. Image collected and cropped by CiteAb from the following open publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-017-3349-7), licensed under a CC-BY license. Not internally tested by R&D Systems.

$Expression of murine Plaur in AT84 cells.In vitro characterization of AT84 cells stably transfected with either empty vector (EV) or a vector containing cDNA encoding murine uPAR (Plaur). A: Western blot analysis of whole cell lysates using a polyclonal anti-murine uPAR antibody (AF534). A total of 7.5 ng of recombinant murine uPAR (rmuPAR) was loaded as a positive control. Re-probing for beta -actin was used as a loading control. B: Western blot analysis of cellular membrane fractions using a polyclonal anti-murine uPAR antibody (AF534). Total protein was measured per sample and 53.5 µg of protein was loaded per lane. A and B: Images were cropped, as no additional bands were detectable. C: FACS analysis of non-permeabilized cells using a polyclonal anti-murine uPAR antibody (AF534). Alexa Fluor 488 anti-goat secondary antibody (A11055) was used as the secondary antibody. The quantified mean Alexa 488 fluorescence signal per cell line is presented in the panel to the right. D and E: Relative Plaur mRNA (uPAR) (D) or Plau mRNA (uPA) (E) expression levels as analysed using RT-qPCR. All expression levels were normalized to the expression of the reference genes Trfc and beta -actin. Error bars represent the standard error of mean (+SEM) and N = 3. One-way ANOVA; *p<0.05. F: Plasminogen-gelatin (upper panel) and gelatin (lower panel) zymography analysis of conditioned medium of cells cultured for 24 hours in SFM. HMW-uPA and mPLM (mouse plasmin) were loaded as positive controls. The images were cropped to size. G: Relative Plasminogen mRNA (Plg) expression levels as analysed using RT-qPCR. Error bars represent standard error of mean (+SEM) and N = 3. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.$

Soluble uPAR shed from mouse OSCC cells induce migration. a-b. Western blot analysis of uPAR protein levels in concentrated conditioned medium from AT84-EV and AT84-uPAR cells. a. Conditioned medium from cells cultured in SFM or SFM containing 10 nM plasminogen (plg) as indicated. The conditioned medium was concentrated from 2 ml to 110 μl for both samples, and deglycosylated using PNGase F. b. Conditioned medium from cells cultured in SFM or in FBSM was concentrated from 2 ml to 350 μl for each sample. A whole cell lysate sample of AT84-uPAR cells was used as a positive control. The two indicated FBSM samples are replicates. Glycosylated suPAR is indicated as suPARglc. c. Non-concentrated conditioned medium from AT84-EV (EV medium) and AT84-uPAR cells (uPAR-medium) was used as an attractant for the AT84-EV cells in a real-time cell migration analysis using the xCELLigence system. N = 3, where each experiment had two technical replicates, and the error bars represent the standard error of mean (±SEM) based on three separate experiments. Image collected and cropped by CiteAb from the following open publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-017-3349-7), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Genetic Strategies Validation. </b>In vivo tongue tumours of EV1 and uPAR1 knock-down cells.IHC uPAR staining and growth pattern of tongue tumours generated from the EV1 and uPAR1 cells containing either shRNA targeting uPAR (EV1-sh and uPAR1-sh), or non-targeting shRNA (uPAR1-NT). A–C: IHC uPAR staining of EV1-sh (A), uPAR1-NT (B) and uPAR1-sh (C) tumours, respectively. Images were recorded at 20x magnifications. D: Representative image depicting the tumour growth pattern at the tumour-stroma interface in hematoxylin/eosin stained EV1-sh. E: The average SI of the uPAR staining in the tumours, with the maximum obtainable score of 9. The error bars shows the +SEM. N = number of tumours; EV1, N = 8/10; EV1-sh, N = 11/16; uPAR1, N = 4/10; uPAR1-NT, N = 3/8; uPAR1-sh, N = 4/16. One-way ANOVA; **p<0.01, *p<0.05. T = Tumour, S = Stroma. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Genetic Strategies Validation. </b>Knock-down of uPAR expression in AT84 cells.shRNA knock-down of Plaur in AT84 cells. A: Flow chart showing the generation of the single cell clones. B: Western blot analysis of whole cell lysates from the single cell clones stably transfected with either shRNA-constructs (shRNA 3 = sh3, shRNA 4 = sh4 or shRNA 5 = sh5) targeting Plaur or constructs containing non-target shRNA (NT) or the empty vector (EV). uPAR was detected using a polyclonal anti-murine uPAR antibody (AF534). Re-probing for beta -actin was used as a loading control. Images were cropped, as no additional bands were detected in the blot. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.

$Expression of murine Plaur in AT84 cells.In vitro characterization of AT84 cells stably transfected with either empty vector (EV) or a vector containing cDNA encoding murine uPAR (Plaur). A: Western blot analysis of whole cell lysates using a polyclonal anti-murine uPAR antibody (AF534). A total of 7.5 ng of recombinant murine uPAR (rmuPAR) was loaded as a positive control. Re-probing for beta -actin was used as a loading control. B: Western blot analysis of cellular membrane fractions using a polyclonal anti-murine uPAR antibody (AF534). Total protein was measured per sample and 53.5 µg of protein was loaded per lane. A and B: Images were cropped, as no additional bands were detectable. C: FACS analysis of non-permeabilized cells using a polyclonal anti-murine uPAR antibody (AF534). Alexa Fluor 488 anti-goat secondary antibody (A11055) was used as the secondary antibody. The quantified mean Alexa 488 fluorescence signal per cell line is presented in the panel to the right. D and E: Relative Plaur mRNA (uPAR) (D) or Plau mRNA (uPA) (E) expression levels as analysed using RT-qPCR. All expression levels were normalized to the expression of the reference genes Trfc and beta -actin. Error bars represent the standard error of mean (+SEM) and N = 3. One-way ANOVA; *p<0.05. F: Plasminogen-gelatin (upper panel) and gelatin (lower panel) zymography analysis of conditioned medium of cells cultured for 24 hours in SFM. HMW-uPA and mPLM (mouse plasmin) were loaded as positive controls. The images were cropped to size. G: Relative Plasminogen mRNA (Plg) expression levels as analysed using RT-qPCR. Error bars represent standard error of mean (+SEM) and N = 3. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.$

<b>Genetic Strategies Validation. </b>Leiomyoma conditioned medium induced uPAR expression.Analysis of uPAR expression induced by the LCM or purified ECM proteins in cultured uPAR knock-down cells. All Western blots were performed on whole cell lysates, and uPAR was detected using the polyclonal anti-murine uPAR antibody (AF534). A: Cells were either cultured in LCM (LM) or serum free medium (SF) for 24 hours. Cells were harvested with sample buffer and re-probing for beta -actin was used as a loading control. B: uPAR1-NT cells were seeded on different ECM protein substrates, incubated for 24 hours and harvested using RIPA buffer. 7.5 µg of total protein was loaded per lane. Equal loading was verified by re-probing for beta -actin. The poloxamer pluronic was used as a no-adhesion control. Col I = Collagen I, Vn = Vitronectin, Fn = Fibronectin, Lm = Laminin, ECL = Entactin, Collagen, Laminin, and FBS = Foetal Bovine Serum. C: Cells cultured in LCM (LM) or serum free medium (SF) for 24 hours were harvested using sample buffer and deglycosylated by PNGase F treatment (+) as indicated. Re-probing for beta -actin was used as a loading control. The three bands detected by the anti-uPAR antibody are labelled 1, 2, and 3, respectively. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.

$Expression of murine Plaur in AT84 cells.In vitro characterization of AT84 cells stably transfected with either empty vector (EV) or a vector containing cDNA encoding murine uPAR (Plaur). A: Western blot analysis of whole cell lysates using a polyclonal anti-murine uPAR antibody (AF534). A total of 7.5 ng of recombinant murine uPAR (rmuPAR) was loaded as a positive control. Re-probing for beta -actin was used as a loading control. B: Western blot analysis of cellular membrane fractions using a polyclonal anti-murine uPAR antibody (AF534). Total protein was measured per sample and 53.5 µg of protein was loaded per lane. A and B: Images were cropped, as no additional bands were detectable. C: FACS analysis of non-permeabilized cells using a polyclonal anti-murine uPAR antibody (AF534). Alexa Fluor 488 anti-goat secondary antibody (A11055) was used as the secondary antibody. The quantified mean Alexa 488 fluorescence signal per cell line is presented in the panel to the right. D and E: Relative Plaur mRNA (uPAR) (D) or Plau mRNA (uPA) (E) expression levels as analysed using RT-qPCR. All expression levels were normalized to the expression of the reference genes Trfc and beta -actin. Error bars represent the standard error of mean (+SEM) and N = 3. One-way ANOVA; *p<0.05. F: Plasminogen-gelatin (upper panel) and gelatin (lower panel) zymography analysis of conditioned medium of cells cultured for 24 hours in SFM. HMW-uPA and mPLM (mouse plasmin) were loaded as positive controls. The images were cropped to size. G: Relative Plasminogen mRNA (Plg) expression levels as analysed using RT-qPCR. Error bars represent standard error of mean (+SEM) and N = 3. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.$

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF534U-100UG
Alexa Fluor 405	AF534V-100UG
Alexa Fluor 488	AF534G-100UG
Alexa Fluor 532	AF534X-100UG
Alexa Fluor 594	AF534T-100UG
Alexa Fluor 647	AF534R-100UG
Alexa Fluor 700	AF534N-100UG
Alexa Fluor 750	AF534S-100UG
Alexa Fluor Plus 405	AF534AFP405-100UG
Alexa Fluor Plus 488	AF534AFP488-100UG
Alexa Fluor Plus 555	AF534AFP555-100UG
Alexa Fluor Plus 594	AF534AFP594-100UG
Alexa Fluor Plus 647	AF534AFP647-100UG
Alexa Fluor Plus 680	AF534AFP680-100UG
Allophycocyanin	AF534A
Biotin	BAF534
CoraFluor 1	AF534CL1
DyLight 350	AF534D
DyLight 405	AF534E
DyLight 488	AF534K
DyLight 550	AF534L
DyLight 594	AF534M
DyLight 650	AF534W
DyLight 680	AF534Y
DyLight 755	AF534Z
FITC	AF534F
HRP	AF534H
Janelia Fluor 525	AF534JF525
Janelia Fluor 549	AF534I
Janelia Fluor 646	AF534J
Janelia Fluor 669	AF534JF669
PE	AF534P
PerCP	AF534C
mFluor Violet 450 SE	AF534MFV450
mFluor Violet 500 SE	AF534MFV500
mFluor Violet 610 SE	AF534MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF534-SP

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(select up to 3 products from this page to compare)

AF807

uPAR Antibody [Unconjugated]

(1 Review)

(14 Publications)

100 μg

AF807

HAF017). Specific bands were detected for uPAR at approximately 30-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of A431 human epithelial carcinoma cell line and Saos-2 human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF807) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF017). Specific bands were detected for uPAR at approximately 30-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A431 human epithelial carcinoma cell line and Saos-2 human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF807) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for uPAR at approximately 30-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

uPAR was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Goat Anti-Human uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF807) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (<a class=

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Simple Western lane view shows lysates of MDA‑MB‑231 human breast cancer cells, loaded at 0.2 mg/mL. A specific band was detected for uPAR at approximately 74 kDa (as indicated) using 20 µg/mL of Goat Anti-Human uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF807). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

807-UK) was serially diluted and captured by Mouse Anti-Human uPAR Monoclonal Antibody (Catalog # MAB807) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF807) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

807-UK) was serially diluted and captured by Mouse Anti-Human uPAR Monoclonal Antibody (Catalog # MAB807) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF807) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF807U-100UG
Alexa Fluor 405	AF807V-100UG
Alexa Fluor 488	AF807G-100UG
Alexa Fluor 532	AF807X-100UG
Alexa Fluor 594	AF807T-100UG
Alexa Fluor 647	AF807R-100UG
Alexa Fluor 700	AF807N-100UG
Alexa Fluor 750	AF807S-100UG
Alexa Fluor Plus 405	AF807AFP405-100UG
Alexa Fluor Plus 488	AF807AFP488-100UG
Alexa Fluor Plus 555	AF807AFP555-100UG
Alexa Fluor Plus 594	AF807AFP594-100UG
Alexa Fluor Plus 647	AF807AFP647-100UG
Alexa Fluor Plus 680	AF807AFP680-100UG
Allophycocyanin	AF807A
Biotin	BAF807
CoraFluor 1	AF807CL1
DyLight 350	AF807D
DyLight 405	AF807E
DyLight 488	AF807K
DyLight 550	AF807L
DyLight 594	AF807M
DyLight 650	AF807W
DyLight 680	AF807Y
DyLight 755	AF807Z
FITC	AF807F
HRP	AF807H
Janelia Fluor 525	AF807JF525
Janelia Fluor 549	AF807I
Janelia Fluor 646	AF807J
Janelia Fluor 669	AF807JF669
PE	AF807P
PerCP	AF807C
mFluor Violet 450 SE	AF807MFV450
mFluor Violet 500 SE	AF807MFV500
mFluor Violet 610 SE	AF807MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF807-SP

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MAB531

uPAR Antibody (109801) [Unconjugated]

(10 Publications)

100 μg

MAB531

MAB006, open histogram) followed by anti-Rat IgG PE-conjugated Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

MAB006, open histogram) followed by anti-Rat IgG PE-conjugated Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins." alt="Mouse whole blood monocytes were stained with Rat Anti-Mouse uPAR Monoclonal Antibody (Catalog # MAB531, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by anti-Rat IgG PE-conjugated Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB531U-100UG
Alexa Fluor 405	FAB531V-100UG
Alexa Fluor 488	FAB531G-100UG
Alexa Fluor 532	FAB531X
Alexa Fluor 594	FAB531T-100UG
Alexa Fluor 647	FAB531R-100UG
Alexa Fluor 700	FAB531N-100UG
Alexa Fluor 750	FAB531S-100UG
Alexa Fluor Plus 405	FAB531AFP405-100UG
Alexa Fluor Plus 488	FAB531AFP488-100UG
Alexa Fluor Plus 555	FAB531AFP555-100UG
Alexa Fluor Plus 594	FAB531AFP594-100UG
Alexa Fluor Plus 647	FAB531AFP647-100UG
Alexa Fluor Plus 680	FAB531AFP680-100UG
Allophycocyanin	FAB531A
Allophycocyanin/Cy7	FAB531APCCY7
Biotin	FAB531B
CoraFluor 1	FAB531CL1
DyLight 350	FAB531D
DyLight 405	FAB531E
DyLight 488	FAB531K
DyLight 550	FAB531L
DyLight 594	FAB531M
DyLight 650	FAB531W
DyLight 680	FAB531Y
DyLight 755	FAB531Z
FITC	FAB531F
HRP	FAB531H
Janelia Fluor 525	FAB531JF525
Janelia Fluor 549	FAB531I
Janelia Fluor 646	FAB531J
Janelia Fluor 669	FAB531JF669
PE/Atto594	FAB531PEATT594
PE/Cy5.5	FAB531PECY55
PE/Cy7	FAB531PECY7
PerCP	FAB531C
Phycoerythrin	FAB531P-100UG
mFluor Violet 450 SE	FAB531MFV450
mFluor Violet 500 SE	FAB531MFV500
mFluor Violet 610 SE	FAB531MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB531-SP
Carrier Free	MAB531-500
Carrier Free	FAB531V-100UG
Carrier Free	FAB531U-100UG
Carrier Free	FAB531T-100UG
Carrier Free	FAB531S-100UG
Carrier Free	FAB531R-100UG
Carrier Free	FAB531N-100UG
Carrier Free	FAB531G-100UG
Carrier Free	FAB531P-100UG

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NBP3-25734

uPAR Antibody (HL2169) - Azide and BSA Free

100 ul

NBP3-25734

<b>Biological Strategies Validation. </b>Western Blot: uPAR Antibody (HL2169) - Azide and BSA Free [NBP3-25734] - Untreated (-) and treated (+) U937 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with uPAR antibody [HL2169] (NBP3-25734) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunocytochemistry/Immunofluorescence: uPAR Antibody (HL2169) - Azide and BSA Free [NBP3-25734] - uPAR antibody [HL2169] detects uPAR protein at cell membrane and cytoplasmic vesicles by immunofluorescent analysis. Sample: U937 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: uPAR stained by uPAR antibody [HL2169] (NBP3-25734) diluted at 1:500. Blue: Fluoroshield with DAPI .

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP3-28067

uPAR Antibody (ATN-658) - Humanized

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

CHO Monoclonal

Host: CHO Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-28067AF350
Alexa Fluor 405	NBP3-28067AF405
Alexa Fluor 488	NBP3-28067AF488
Alexa Fluor 532	NBP3-28067AF532
Alexa Fluor 594	NBP3-28067AF594
Alexa Fluor 647	NBP3-28067AF647
Alexa Fluor 700	NBP3-28067AF700
Alexa Fluor 750	NBP3-28067AF750
Alexa Fluor Plus 405	NBP3-28067AFP405
Alexa Fluor Plus 488	NBP3-28067AFP488
Alexa Fluor Plus 555	NBP3-28067AFP555
Alexa Fluor Plus 594	NBP3-28067AFP594
Alexa Fluor Plus 647	NBP3-28067AFP647
Alexa Fluor Plus 680	NBP3-28067AFP680
Allophycocyanin	NBP3-28067APC
Biotin	NBP3-28067B
CoraFluor 1	NBP3-28067CL1
DyLight 350	NBP3-28067UV
DyLight 405	NBP3-28067V
DyLight 488	NBP3-28067G
DyLight 550	NBP3-28067R
DyLight 594	NBP3-28067DL594
DyLight 650	NBP3-28067C
DyLight 680	NBP3-28067FR
DyLight 755	NBP3-28067IR
FITC	NBP3-28067F
HRP	NBP3-28067H
Janelia Fluor 525	NBP3-28067JF525
Janelia Fluor 549	NBP3-28067JF549
Janelia Fluor 646	NBP3-28067JF646
Janelia Fluor 669	NBP3-28067JF669
PE	NBP3-28067PE
PerCP	NBP3-28067PCP
mFluor Violet 450 SE	NBP3-28067MFV450
mFluor Violet 500 SE	NBP3-28067MFV500
mFluor Violet 610 SE	NBP3-28067MFV610

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MAB11694

uPAR Antibody (1065526) [Unconjugated]

100 μg

MAB11694

NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm of MDA-MB-231 cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

uPAR was detected in immersion fixed MDA‑MB‑231 human breast cancer cell line (Positive) and absent in U266 human myeloma cell line (Negative) using Mouse Anti-Human uPAR Monoclonal Antibody (Catalog # MAB11694) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm of MDA-MB-231 cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="uPAR was detected in immersion fixed MDA‑MB‑231 human breast cancer cell line (Positive) and absent in U266 human myeloma cell line (Negative) using Mouse Anti-Human uPAR Monoclonal Antibody (Catalog # MAB11694) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm of MDA-MB-231 cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB11694U-100UG
Alexa Fluor 405	FAB11694V-100UG
Alexa Fluor 488	FAB11694G-100UG
Alexa Fluor 532	FAB11694X-100UG
Alexa Fluor 594	FAB11694T-100UG
Alexa Fluor 647	FAB11694R-100UG
Alexa Fluor 700	FAB11694N-100UG
Alexa Fluor 750	FAB11694S-100UG
Alexa Fluor Plus 405	FAB11694AFP405-100UG
Alexa Fluor Plus 488	FAB11694AFP488-100UG
Alexa Fluor Plus 555	FAB11694AFP555-100UG
Alexa Fluor Plus 594	FAB11694AFP594-100UG
Alexa Fluor Plus 647	FAB11694AFP647-100UG
Alexa Fluor Plus 680	FAB11694AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB11694-SP

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NBP2-14711

uPAR Antibody - BSA Free

0.1 ml

NBP2-14711

Immunohistochemistry-Paraffin: uPAR Antibody [NBP2-14711] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.

Immunohistochemistry-Paraffin: uPAR Antibody [NBP2-14711] - Staining of human skeletal muscle shows no cytoplasmic positivity as expected.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-14711-25ul

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NBP2-62789

uPAR Antibody (IIIB6)

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-62800

uPAR Antibody (IIIB11)

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP3-30507

uPAR Antibody - BSA Free

100 μg

NBP3-30507

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP3-29589

uPAR Antibody - BSA Free

100 μg

NBP3-29589

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Has Sample Size

Promotional Item

Species Glossary

uPAR Antibodies

uPAR Antibody (62022) [Unconjugated]

uPAR Antibody [Unconjugated]

uPAR Antibody [Unconjugated]

uPAR Antibody (109801) [Unconjugated]

uPAR Antibody (HL2169) - Azide and BSA Free

uPAR Antibody (ATN-658) - Humanized

uPAR Antibody (1065526) [Unconjugated]

uPAR Antibody - BSA Free

uPAR Antibody (IIIB6)

uPAR Antibody (IIIB11)

uPAR Antibody - BSA Free

uPAR Antibody - BSA Free