ULBP-1 Antibody (170818) [Phycoerythrin]

MOLT-4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human ULBP-1 PE-conjugated Monoclonal Antibody (Catalog # FAB1380P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 170818
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

ULBP-1 Antibody (170818) [Phycoerythrin] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with human ULBP-1
• Specificity: Stains human ULBP-1 cell transfectants. It does not stain cells transfected with ULBP-2 or ULBP-3.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ULBP1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ULBP-1 Antibody (170818) [Phycoerythrin]

• ALCAN-beta
• MULT-1
• N2DL1
• N2DL-1
• NKG2D ligand 1
• NKG2DL
• NKG2DL1
• RAET1I
• RAET1Ialcan-beta
• Retinoic acid early transcript 1I
• UL16 binding protein 1
• UL16-binding protein 1
• ULBP1
• ULBP-1

Background

ULBP-1 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-1 has also been described under the names RaeT1I (retinoic acid early transcript), ALCAN-beta, and NKG2DL1. The name ULBP-1 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-1 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30-95%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. They are anchored to the membrane via a GPI-linkage. ULBP-1 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8⁺ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. ULBP-1 is expressed on some tumor cells and has been implicated in tumor surveillance (1-8).

1. Cosman, D. et al. (2001) Immunity 14:123.
2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
3. Sutherland, C. et al. (2002) J. Immunol. 168:671.
4. Steinle, A. et al. (2001) Immunogenetics 53:279.
5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185
6. Pende, D. et al. (2002) Cancer Res. 62:6178.
7. Radosavljevic, M. et al. (2002) Genomics 79:114
8. NKG2D and its Ligands, 2002; www.RnDSystems.com.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ULBP-1 Antibody (FAB1380P)(31)

Publications using FAB1380P

• Sánchez-Cerrillo, I;Agudo-Lera, M;Popova, O;Tsukalov, I;Calvet-Mirabent, M;de Los Santos, I;García-Fraile, L;Fuentes, P;Delgado-Arévalo, C;Alcain, J;Sánchez-Gaona, N;Grau-Expósito, J;Lázaro-Díez, M;Muñoz-Calleja, C;Alfranca, A;Genescà, M;Prado, JG;Vrbanac, V;Balazs, A;Buzón, MJ;Toribio, ML;Muñoz-Fernández, MA;Sánchez-Madrid, F;Martín-Gayo, E; Combined dendritic cell and anti-TIGIT immunotherapy potentiates adaptive NK cells against HIV-1 EMBO molecular medicine 2025-06-05 [PMID: 40473838] (Flow Cytometry, Human)
• He, X;Wang, L;Zeng, W;Wang, Y;Chen, N;Yang, P;Ti, A;Zhang, Q;Shao, Y;Wang, M;Huang, Z;Zhang, X;Xu, M;Liang, L;Wang, X;Ding, X;Zhu, T;Zhang, P;Pan, Z;Yang, F;Zhou, Y;Mo, G;Hu, J;Yue, Y;Hu, J;Deng, Y;Ho, T;Church, GM;Hu, Y;Huang, H;Yang, L; Regulatable C-X-C chemokine receptor type 4 in iPSC-derived NK cells improves bone marrow chemotaxis and targeting resident tumor Trends in biotechnology 2025-04-30 [PMID: 40312161] (Flow Cytometry, Human)
• Li, YR;Fang, Y;Niu, S;Zhu, Y;Chen, Y;Lyu, Z;Zhu, E;Tian, Y;Huang, J;Rezek, V;Kitchen, S;Hsiai, T;Zhou, JJ;Wang, P;Chai-Ho, W;Park, S;Seet, CS;Oliai, C;Yang, L; Allogeneic CD33-directed CAR-NKT cells for the treatment of bone marrow-resident myeloid malignancies Nature communications 2025-02-01 [PMID: 39893165] (Flow Cytometry, Human)
• Lee, S;Chae, SJ;Jang, IH;Oh, SC;Kim, SM;Lee, SY;Kim, JH;Ko, J;Kim, HJ;Song, IC;Kim, JK;Kim, TD; B7H6 is the predominant activating ligand driving natural killer cell-mediated killing in patients with liquid tumours: evidence from clinical, in silico, in vitro, and in vivo studies EBioMedicine 2024-11-22 [PMID: 39579618] (FACS, Human)
• Jones, AB;Tuy, K;Hawkins, CC;Quinn, CH;Saad, J;Gary, SE;Beierle, EA;Ding, L;Rochlin, KM;Lamb, LS;Hjelmeland, AB; Temozolomide and the PARP Inhibitor Niraparib Enhance Expression of Natural Killer Group 2D Ligand ULBP1 and Gamma-Delta T Cell Cytotoxicity in Glioblastoma Cancers 2024-08-15 [PMID: 39199623] (Flow Cytometry, Xenograft)
• Pascual-Pasto, G;McIntyre, B;Hines, MG;Giudice, AM;Garcia-Gerique, L;Hoffmann, J;Mishra, P;Matlaga, S;Lombardi, S;Shraim, R;Schürch, PM;Yarmarkovich, M;Hofmann, TJ;Alikarami, F;Martinez, D;Tsang, M;Gil-de-Gómez, L;Spear, TT;Bernt, KM;Wolpaw, AJ;Dimitrov, DS;Li, W;Bosse, KR; CAR T-cell-mediated delivery of bispecific innate immune cell engagers for neuroblastoma Nature communications 2024-08-21 [PMID: 39164224] (Flow Cytometry, Human)
• Li, YR;Zhou, Y;Yu, J;Kim, YJ;Li, M;Lee, D;Zhou, K;Chen, Y;Zhu, Y;Wang, YC;Li, Z;Yu, Y;Dunn, ZS;Guo, W;Cen, X;Husman, T;Bajpai, A;Kramer, A;Wilson, M;Fang, Y;Huang, J;Li, S;Zhou, Y;Zhang, Y;Hahn, Z;Zhu, E;Ma, F;Pan, C;Lusis, AJ;Zhou, JJ;Seet, CS;Kohn, DB;Wang, P;Zhou, XJ;Pellegrini, M;Puliafito, BR;Larson, SM;Yang, L; Generation of allogeneic CAR-NKT cells from hematopoietic stem and progenitor cells using a clinically guided culture method Nature biotechnology 2024-05-14 [PMID: 38744947] (Flow Cytometry, Human)
• Fisher, JG;Doyle, ADP;Graham, LV;Sonar, S;Sale, B;Henderson, I;Del Rio, L;Johnson, PWM;Landesman, Y;Cragg, MS;Forconi, F;Walker, CJ;Khakoo, SI;Blunt, MD; XPO1 inhibition sensitises CLL cells to NK cell mediated cytotoxicity and overcomes HLA-E expression Leukemia 2023-08-01 [PMID: 37528310] (Flow Cytometry, Human)
• Koh, EK;Lee, HR;Son, WC;Park, GY;Bae, J;Park, YS; Antitumor effects of NK cells expanded by activation pre?processing of autologous feeder cells before irradiation in colorectal cancer Oncology letters 2023-06-01 [PMID: 37153058] (Flow Cytometry, Human)
• NL de Vries, J van de Haa, V Veninga, M Chalabi, ME Ijsselstei, M van der Pl, J van den Bu, D Ruano, JG van den Be, JB Haanen, LJ Zeverijn, BS Geurts, GF de Wit, TW Battaglia, H Gelderblom, HMW Verheul, TN Schumacher, LFA Wessels, F Koning, NFCC de Miranda, EE Voest gammadelta T cells are effectors of immunotherapy in cancers with HLA class I defects Nature, 2023-01-11;613(7945):743-750. 2023-01-11 [PMID: 36631610] (Flow Cytometry, Human)
• Y Murayama, Y Kasahara, N Kubo, C Shin, M Imamura, N Oike, T Ariizumi, A Saitoh, M Baba, T Miyazaki, Y Suzuki, Y Ling, S Okuda, K Mihara, A Ogose, H Kawashima, C Imai NKp44-based chimeric antigen receptor effectively redirects primary T cells against synovial sarcoma Translational Oncology, 2022-08-20;25(0):101521. 2022-08-20 [PMID: 35998437] (Flow Cytometry, Human)
• HY Wu, KX Li, WY Pan, MQ Guo, DZ Qiu, YJ He, YH Li, YX Huang Venetoclax enhances NK cell killing sensitivity of AML cells through the NKG2D/NKG2DL activation pathway International immunopharmacology, 2022-01-06;104(0):108497. 2022-01-06 [PMID: 34999394] (Flow Cytometry, Human)
• Juan Fan, Jiawei Shi, Yong Zhang, Junwei Liu, Chenyi An, Huaying Zhu et al. NKG2D discriminates diverse ligands through selectively mechano‐regulated ligand conformational changes The EMBO Journal 1/17/2022 [PMID: 34913508]
• T Seki, Y Shimizu, K Ishii, Y Takahama, K Kato, T Yano NK Cells Can Preferentially Target Prostate Cancer Stem-like Cells via the TRAIL/DR5 Signaling Pathway Biomolecules, 2021-11-16;11(11):. 2021-11-16 [PMID: 34827699] (Flow Cytometry, Human)
• R Fu, F Yu, W Wu, J Liu, J Li, F Guo, L Xu, F Wang, X Cui Bufalin enhances the killing efficacy of NK cells against hepatocellular carcinoma by inhibiting MICA shedding International immunopharmacology, 2021-10-19;101(0):108195. 2021-10-19 [PMID: 34678691] (Flow Cytometry, Human)
• MV Martínez-S, JL Fuster, JA Campillo, AM Galera, M Bermúdez-C, ME Llinares, E Ramos-Elba, JF Pascual-Gá, AM Fita, H Martínez-B, JA Galián, L Gimeno, M Muro, A Minguela Expression of NK Cell Receptor Ligands on Leukemic Cells Is Associated with the Outcome of Childhood Acute Leukemia Cancers, 2021-05-11;13(10):. 2021-05-11 [PMID: 34064810] (Flow Cytometry, Human)
• YH Liang, JH Tsai, YM Cheng, KY Chan, WL Hsu, CC Lee, KH Chen, KH Yeh Chemotherapy agents stimulate dendritic cells against human colon cancer cells through upregulation of the transporter associated with antigen processing Scientific Reports, 2021-04-27;11(1):9080. 2021-04-27 [PMID: 33907276] (Flow Cytometry, Western Blot, Human)
• Sofie H. Møller, Maiken Mellergaard, Mikkel Madsen, Amaia V. Bermejo, Stine D. Jepsen, Marie H. Hansen et al. Cytoplasmic Citrate Flux Modulates the Immune Stimulatory NKG2D Ligand MICA in Cancer Cells Frontiers in Immunology 8/11/2020 [PMID: 32849657]
• RI Høgh, A Droujinine, SH Møller, SD Jepsen, M Mellergaar, L Andresen, S Skov Fumarate Upregulates Surface Expression of ULBP2/ULBP5 by Scavenging Glutathione Antioxidant Capacity J. Immunol., 2020-03-06;0(0):. 3/6/2020 [PMID: 32144161]
• D Suzuki, C Flahou, N Yoshikawa, I Stirblyte, Y Hayashi, A Sawaguchi, M Akasaka, S Nakamura, N Higashi, H Xu, T Matsumoto, K Fujio, MG Manz, A Hotta, H Takizawa, K Eto, N Sugimoto iPSC-Derived Platelets Depleted of HLA Class I Are Inert to Anti-HLA Class I and Natural Killer Cell Immunity Stem Cell Reports, 2019-12-26;0(0):. 2019-12-26 [PMID: 31883921] (Flow Cytometry, Human)
• M Chollat-Na, T Ben Safta-, D Haferssas, G Meurice, S Chouaib, J Thiery The pharmalogical reactivation of p53 function improves breast tumor cell lysis by granzyme B and NK cells through induction of autophagy Cell Death Dis, 2019-09-20;10(10):695. 2019-09-20 [PMID: 31541080] (Flow Cytometry, Human)
• Y Han, W Xie, DG Song, DJ Powell Control of triple-negative breast cancer using ex vivo self-enriched, costimulated NKG2D CAR T cells J Hematol Oncol, 2018-07-06;11(1):92. 2018-07-06 [PMID: 29980239] (CAR-T (Flow Cytometry), Human)
• Ryoji Kamei, Kiyoshi Yoshimura, Shigefumi Yoshino, Moeko Inoue, Tetsuhiko Asao, Masanori Fuse et al. Expression levels of UL16 binding protein 1 and natural killer group 2 member D affect overall survival in patients with gastric cancer following gastrectomy Oncology Letters 11/6/2017 [PMID: 29391893]
• Ryoji Kamei, Kiyoshi Yoshimura, Shigefumi Yoshino, Moeko Inoue, Tetsuhiko Asao, Masanori Fuse et al. Expression levels of UL16 binding protein 1 and natural killer group 2 member D affect overall survival in patients with gastric cancer following gastrectomy Oncology Letters 2017-11-06 [PMID: 29391893] (Flow Cytometry, Human)
• JRM Van Audena, J De Waele, E Marcq, J Van Loenho, E Lion, JMJ Van den Be, R Jesenofsky, A Masamune, G Roeyen, P Pauwels, F Lardon, M Peeters, ELJ Smits Interleukin-15 stimulates natural killer cell-mediated killing of both human pancreatic cancer and stellate cells Oncotarget, 2017-01-01;0(0):. 2017-01-01 [PMID: 28615524] (Flow Cytometry, Human)
• Histone Deacetylase Inhibitors Enhance CD4 T Cell Susceptibility to NK Cell Killing but Reduce NK Cell Function PLoS Pathog, 2016-08-16;12(8):e1005782. 2016-08-16 [PMID: 27529554] (Flow Cytometry, Human)
• Guranda Chitadze, Marcus Lettau, Stefanie Luecke, Ting Wang, Ottmar Janssen, Daniel Fürst et al. NKG2D- and T-cell receptor-dependent lysis of malignant glioma cell lines by human gamma δ T cells: Modulation by temozolomide and A disintegrin and metalloproteases 10 and 17 inhibitors OncoImmunology 4/2/2016 [PMID: 27141377]
• Decitabine enhances anti-CD33 monoclonal antibody BI 836858-mediated natural killer ADCC against AML blasts Blood, 2016-03-24;127(23):2879-89. 2016-03-24 [PMID: 27013443] (Flow Cytometry, Human)
• Parrish C, Scott G, Migneco G, Scott K, Steele L, Ilett E, West E, Hall K, Selby P, Buchanan D, Varghese A, Cragg M, Coffey M, Hillmen P, Melcher A, Errington-Mais F Oncolytic reovirus enhances rituximab-mediated antibody-dependent cellular cytotoxicity against chronic lymphocytic leukaemia. Leukemia, 2015-03-27;29(9):1799-810. 2015-03-27 [PMID: 25814029] (Flow Cytometry, Human)
• Wang , Ruipeng, Sun , Peter D Natural killer cell-mediated shedding of ULBP2. PLoS ONE, 2014-03-10;9(3):e91133. 2014-03-10 [PMID: 24614922] (Flow Cytometry, Human)
• Sandra A. Jacobs, Jeroen Plessers, Jef Pinxteren, Valerie D. Roobrouck, Catherine M. Verfaillie, Stefaan W. Van Gool Mutual Interaction between Human Multipotent Adult Progenitor Cells and NK Cells Cell Transplantation 9/1/2014 [PMID: 23562064]

Bioinformatics

• Gene Symbol: ULBP1