UGRP1/SCGB3A2 Antibody (381707)

Basal cell (BC) subpopulations other than BC-1 and BC-2 are relatively rare and have no consistent spatial distribution in the adult tracheal epithelium.Representative tile scan pictures of adult mouse tracheal sections immunostained for KRT5, TRP63 and representative markers of Basal-proliferating (A), Basal-squamous (B), Basal-mesenchyme-like (C), and Basal-secretory (D). High magnification images are representative of specific BC subpopulations depicted in boxed areas along the anterior-posterior or dorsal-ventral axis of the tracheal epithelium. Scale bars: large panels of overview: 200 μm; small panels: 10 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36178196), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB
• Clone: 381707
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

UGRP1/SCGB3A2 Antibody (381707) Summary

• Immunogen: E. coli-derived recombinant mouse UGRP1/SCGB3A2 isoform C
  Leu22-Leu139
  Accession # Q920H1
• Specificity: Detects mouse UGRP1/SCGB3A2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human UGRP1 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: SCGB3A2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for UGRP1/SCGB3A2 Antibody (381707)

• LU103
• Pneumo secretory protein 1
• PnSP-1
• PNSP1secretoglobin family 3A member 2
• SCGB3A2
• secretoglobin, family 3A, member 2
• UGRP1
• UGRP1pnSP-1
• Uteroglobin-related protein 1

Background

UGRP1 (also secretglobin gene family 3A2/SCGB3A2) is an 18 kDa, secreted nonglycosylated polypeptide that is a member of the Uteroglonin family, UGRP subfamily. Mouse UGRP1(isoform A) circulates as a 37 kDa homodimer. Its mature form is 70 amino acids in length. There are two large alternate splice forms termed B and C. UGRP1B is a secreted homodimer that shows a 22 aa insert between aa 84 and 85 of the short form. UGRP1C contains a 55 aa substitution for the C-terminal seven aa of the short form, predicting a 118 aa secreted form. The common portion of mature mouse UGRP1 (isoforms A, B and C) shares 86% and 81% aa identity with rat and human UGRP1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UGRP1/SCGB3A2 Antibody (MAB3465)(4)

Publications using MAB3465

• He, H;Bell, SM;Davis, AK;Zhao, S;Sridharan, A;Na, CL;Guo, M;Xu, Y;Snowball, J;Swarr, DT;Zacharias, WJ;Whitsett, JA; PRDM3/16 regulate chromatin accessibility required for NKX2-1 mediated alveolar epithelial differentiation and function Nature communications 2024-09-16 [PMID: 39284798] (Immunohistochemistry, Transgenic Mouse)
• Yizhuo Zhou, Ying Yang, Lihao Guo, Jun Qian, Jian Ge, Debora Sinner et al. Airway basal cells show regionally distinct potential to undergo metaplastic differentiation eLife 2022-09-30 [PMID: 36178196]
• Carlos S, Helu L, Jun Q et al. Prominin 1 and Notch regulate ciliary length and dynamics in multiciliated cells of the airway epithelium. iScience. 2022-07-14 [PMID: 35942101]
• Yin W, Liontos A, Koepke J et al. An essential function for autocrine Hedgehog signaling in epithelial proliferation and differentiation in the trachea bioRxiv 1/1/2022 [PMID: 35112129]

Bioinformatics

• Gene Symbol: SCGB3A2
• Uniprot:
  • Q920H1()