UGP2 Antibody (7H1)

Western Blot: UGP2 Antibody (7H1) [NBP2-75695] - Analysis of UGP2 on human liver tissue lysate using anti-UGP2 antibody at 1/200 dilution.

Immunohistochemistry-Paraffin: UGP2 Antibody (7H1) [NBP2-75695] - Analysis of paraffin-embedded rat skeletal muscle tissue using anti-UGP2 antibody. Counter stained with hematoxylin.

Flow Cytometry: UGP2 Antibody (7H1) [NBP2-75695] - Analysis of LOVO cells with UGP2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: UGP2 Antibody (7H1) [NBP2-75695] - Analysis of paraffin-embedded human liver tissue using anti-UGP2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: UGP2 Antibody (7H1) [NBP2-75695] - Analysis of paraffin-embedded mouse heart tissue using anti-UGP2 antibody. Counter stained with hematoxylin.

Product Discontinued

UGP2 Antibody (7H1) Summary

• Immunogen: Synthetic peptide within Human UGP2 aa 61-110 / 508. (SwissProt: Q16851 Human; SwissProt: Q91ZJ5 Mouse)
• Localization: Cytoplasm.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: UGP2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein G purified

Alternate Names for UGP2 Antibody (7H1)

• EC 2.7.7.9
• pHC379
• UDPG
• UDP-glucose diphosphorylase
• UDP-glucose pyrophosphorylase 2
• UDP-glucose pyrophosphorylase
• UDPGP
• UDPGP2
• UGP1
• UGPase 2
• UGPase
• UGPP2
• uridyl diphosphate glucose pyrophosphorylase 2
• UTP-glucose-1-phosphate uridyltransferase
• UTP--glucose-1-phosphate uridylyltransferase 2
• UTP--glucose-1-phosphate uridylyltransferase

Background

The enzyme encoded by this gene is an important intermediary in mammalian carbohydrate interconversions. It transfers a glucose moiety from glucose-1-phosphate to MgUTP and forms UDP-glucose and MgPPi. In liver and muscle tissue, UDP-glucose is a direct precursor of glycogen; in lactating mammary gland it is converted to UDP-galactose which is then converted to lactose. The eukaryotic enzyme has no significant sequence similarity to the prokaryotic enzyme. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UGP2