P2Y14/GPR105 Antibody (5A3F1) - BSA Free

Western Blot: P2Y14/GPR105 Antibody (5A3F1) [NBP2-61753] - Analysis using P2RY14 mAb against human P2RY14 (AA: extra mix) recombinant protein. (Expected MW is 11.5 kDa)

Immunocytochemistry/Immunofluorescence: P2Y14/GPR105 Antibody (5A3F1) [NBP2-61753] - Analysis of Hela cells using P2RY14 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry: P2Y14/GPR105 Antibody (5A3F1) [NBP2-61753] - Analysis of paraffin-embedded rectum cancer tissues using P2RY14 mouse mAb with DAB staining.

Flow Cytometry: P2Y14/GPR105 Antibody (5A3F1) [NBP2-61753] - Analysis of Hela cells using P2RY14 mouse mAb (green) and negative control (red).

Western Blot: P2Y14/GPR105 Antibody (5A3F1) [NBP2-61753] - Analysis using P2RY14 mAb against HEK293 (1) and P2RY14 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry: P2Y14/GPR105 Antibody (5A3F1) [NBP2-61753] - Analysis of paraffin-embedded lung cancer tissues using P2RY14 mouse mAb with DAB staining.

ELISA: P2Y14/GPR105 Antibody (5A3F1) [NBP2-61753] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
• Clone: 5A3F1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

P2Y14/GPR105 Antibody (5A3F1) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human P2RY14 (AA: extra mix) expressed in E. Coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: P2RY14
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for P2Y14/GPR105 Antibody (5A3F1) - BSA Free

• G protein coupled receptor for UDP-glucose
• GPR105
• G-protein coupled receptor 105
• KIAA0001G protein-coupled receptor 105
• P2RY14
• P2Y purinoceptor 14
• P2Y(14) receptor
• P2Y14 receptor
• P2Y14
• purinergic receptor P2Y, G-protein coupled, 14
• UDP-glucose receptor

Background

The product of this gene belongs to the family of G-protein coupled receptors, which contains several receptor subtypes with different pharmacological selectivity for various adenosine and uridine nucleotides. This receptor is a P2Y purinergic receptor for UDP-glucose and other UDP-sugars coupled to G-proteins. It has been implicated in extending the known immune system functions of P2Y receptors by participating in the regulation of the stem cell compartment, and it may also play a role in neuroimmune function. Two transcript variants encoding the same protein have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: P2RY14