UCP4 Antibody

Product Discontinued

UCP4 Antibody Summary

• Immunogen: Synthetic peptide corresponding to the amino acids 271 - 283 of the native molecule conjugated to Keyhole limpet Haemocyanin. Peptide Sequence K-S-S-T-D-C-L-I-Q-A-V-Q-G
• Specificity: UNCOUPLING PROTEIN 4
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC25A27
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: In reduced blots on bovine brown adipose tissue homogenates, this antibody recognises specific bands at 34 & 44kD and other bands at 50 & 60 kD that can be blocked with KLH.

Reactivity Notes

Chemical

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Phosphate buffered saline
• Preservative: 0.09% Sodium Azide
• Concentration: 5.0 mg/ml
• Purity: Protein G purified

Alternate Names for UCP4 Antibody

• FLJ33552
• mitochondrial uncoupling protein 4
• Solute carrier family 25 member 27
• solute carrier family 25, member 27
• UCP 4
• UCP4uncoupling protein 4

Background

Mitochondrial uncoupling proteins (UCPs) create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. They may play a role in thermoregulatory heat production and metabolism in brain. They are found in adult and fetal brain and are present in most of the brain tissues, with low levels in spinal chord, corpus callosum and substantia nigra. They are specifically modulated by various environmental conditions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UCP4 Antibody (NB100-66495)(1)

Publication using NB100-66495

• Alves-Bezerra M, Cosentino-Gomes D, Vieira LP et al. Identification of uncoupling protein 4 from the blood-sucking insect Rhodnius prolixus and its possible role on protection against oxidative stress. Insect Biochem. Mol. Biol. 2014-04-16 [PMID: 24746771] (WB, Insect (Rhodnius prolixus))

Bioinformatics

• Gene Symbol: SLC25A27
• Entrez:
  • 9481(Human)
• Uniprot:
  • O95847(Human)