UBXN1 Antibody - BSA Free

Western Blot: UBXN1 Antibody [NBP2-20795] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE gel, diluted at 1:3000.

Immunocytochemistry/Immunofluorescence: UBXN1 Antibody [NBP2-20795] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: UBXN1 Antibody [NBP2-20795] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.

Western Blot: UBXN1 Antibody [NBP2-20795] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 10% SDS PAGE gel, diluted at 1:1000.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

UBXN1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit UBXN1 Antibody - BSA Free (NBP2-20795) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human UBXN1. The exact sequence is proprietary.
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBXN1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for UBXN1 Antibody - BSA Free

• 2B28
• LOC51035
• SAKS1UBA/UBX 33.3 kDa protein
• SAPK substrate protein 1
• UBX domain protein 1
• UBX domain-containing protein 1
• UBXD10

Background

Ubiquitin-binding protein that interacts with the BRCA1-BARD1 heterodimer, and regulates its activity. Specifically binds 'Lys-6'-linked polyubiquitin chains. Interaction with autoubiquitinated BRCA1, leads to inhibit the E3 ubiquitin-protein ligase activity of the BRCA1-BARD1 heterodimer. Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: UBXN1