UBTF Antibody (2D8)

Western Blot: UBTF Antibody (2D8) [H00007343-M04] - Analysis of UBTF expression in HepG2 (Cat # L019V1).

Immunocytochemistry/Immunofluorescence: UBTF Antibody (2D8) [H00007343-M04] - Analysis of monoclonal antibody to UBTF on HepG2 cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: UBTF Antibody (2D8) [H00007343-M04] - Analysis of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml

Western Blot: UBTF Antibody (2D8) [H00007343-M04] - Analysis of UBTF expression in PC-12 (Cat # L012V1).

Western Blot: UBTF Antibody (2D8) [H00007343-M04] - Analysis of UBTF expression in NIH/3T3 (Cat # L018V1).

ELISA: UBTF Antibody (2D8) [H00007343-M04] - Detection limit for recombinant GST tagged UBTF is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P
• Clone: 2D8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

UBTF Antibody (2D8) Summary

• Immunogen: UBTF (NP_055048, 551 a.a. ~ 650 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PPAATNSSKKMKFQGEPKKPPMNGYQKFSQELLSNGELNHLPLKERMVEIGSRWQRISQSQKEHYKKLAEEQQKQYKVHLDLWVKSLSPQDRAAYKEYIS
• Specificity: UBTF - upstream binding transcription factor, RNA polymerase I (2D8)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: UBTF
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for UBTF Antibody (2D8)

• 90-KDa Nucleolus Organizer Region Autoantigen
• Autoantigen NOR-90
• NOR-90
• nucleolar transcription factor 1
• UBF
• UBF1
• UBF-1
• UBF2
• UBFUBF-1
• upstream binding transcription factor, RNA polymerase I
• Upstream-binding factor 1

Background

Upstream binding factor (UBF) is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1 (a complex of TBP (MIM 600075) and multiple TBP-associated factors or 'TAFs'). Two UBF polypeptides, of 94 and 97 kD, exist in the human (Bell et al., 1988 [PubMed 3413483]). UBF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes (Jantzen et al., 1990 [PubMed 2330041]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UBTF Antibody (H00007343-M04)(1)

Publication using H00007343-M04

• Lum KKY Integrating Virology and Proteomics to Comprehend Mechanisms of Innate Immune Signaling During Herpesvirus Infection Thesis (WB, ICC/IF, Human)

Bioinformatics

• Gene Symbol: UBTF
• Entrez:
  • 7343(Human)
• OMIM:
  • 600673(Human)
• Uniprot:
  • NP_055048(Human)