UBPY/USP8 Antibody

Western blot shows lysates of U2OS human osteosarcoma cell line and KG-1 human acute myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human USP8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7735) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for USP8 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

UBPY/USP8 Antibody Summary

• Immunogen: E. coli-derived recombinant human USP8
  Met1-Thr1118
  Accession # P40818
• Specificity: Detects human USP8 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human USP2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: USP8
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for UBPY/USP8 Antibody

• Deubiquitinating enzyme 8
• EC 3.1.2.15
• EC 3.4.19.12
• HumORF8
• KIAA0055hUBPy
• MGC129718
• ubiquitin carboxyl-terminal hydrolase 8
• Ubiquitin isopeptidase Y
• ubiquitin specific peptidase 8
• ubiquitin specific protease 8
• ubiquitin thioesterase 8
• Ubiquitin thiolesterase 8
• Ubiquitin-specific-processing protease 8
• UBPY
• UBPYFLJ34456
• USP8

Background

USP8 (Ubiquitin-Specific-Processing protein 8; also UBPY and ubiquitin carboxyterminal hydrolase 8) is a 125-130 kDa member of the peptidase C19 family of proteins. It is a widely expressed deubiquitinating enzyme that regulates the internalization and trafficking of transmembrane growth factor receptors. Ubiquitinated TKRs are typically targeted for internalization and degradation, and USP8 appears to block this activity. Conversely, USP8 has been shown to have opposite effects on GPCR activity. Here, it accelerates ubiquitinated chemokine receptor (CXCR4) internalization and subsequent inactivation, notably through a deubiquitination-independent mechanism. Human USP8 is 1118 amino acids (aa) in length. It contains one MIT domain that mediates endosomal trafficking (aa 33-116), a rhodanese domain that binds RNF proteins (aa 184-305), an SH3 domain binding sequence (aa 405-413), and a C-terminal catalytic domain that hydrolyses ubiquitin adducts (aa 776‑1106). USP8 is phosphorylated at Ser702, 705, 784 and 787. There is one oncogenic recombination isoform that fuses the p85 beta -subunit of PI3 kinase to the C‑terminal 363 aa of USP8, beginning with Pro746. Full-length human and mouse USP8 share 82% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UBPY/USP8 Antibody (AF7735)(1)

Publication using AF7735

• Manuel Nico Dold, Xiulin Ng, Claudia Alber, Ian Edward Gentle, Georg Häcker, Arnim Weber The deubiquitinase Usp27x as a novel regulator of cFLIPL protein expression and sensitizer to death-receptor-induced apoptosis Apoptosis 2022-02-01 [PMID: 35044632]

Bioinformatics

• Gene Symbol: USP8
• Uniprot:
  • P40818(Human)