UBPY/USP8 Antibody

Images

 
Western blot shows lysates of U2OS human osteosarcoma cell line and KG‑1 human acute myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human USP8 Antigen Affinity-purified Polyclonal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

UBPY/USP8 Antibody Summary

Immunogen
E. coli-derived recombinant human USP8
Met1-Thr1118
Accession # P40818
Specificity
Detects human USP8 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human USP2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
USP8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for UBPY/USP8 Antibody

  • Deubiquitinating enzyme 8
  • EC 3.1.2.15
  • EC 3.4.19.12
  • HumORF8
  • KIAA0055hUBPy
  • MGC129718
  • ubiquitin carboxyl-terminal hydrolase 8
  • Ubiquitin isopeptidase Y
  • ubiquitin specific peptidase 8
  • ubiquitin specific protease 8
  • ubiquitin thioesterase 8
  • Ubiquitin thiolesterase 8
  • Ubiquitin-specific-processing protease 8
  • UBPY
  • UBPYFLJ34456
  • USP8

Background

USP8 (Ubiquitin-Specific-Processing protein 8; also UBPY and ubiquitin carboxyterminal hydrolase 8) is a 125-130 kDa member of the peptidase C19 family of proteins. It is a widely expressed deubiquitinating enzyme that regulates the internalization and trafficking of transmembrane growth factor receptors. Ubiquitinated TKRs are typically targeted for internalization and degradation, and USP8 appears to block this activity. Conversely, USP8 has been shown to have opposite effects on GPCR activity. Here, it accelerates ubiquitinated chemokine receptor (CXCR4) internalization and subsequent inactivation, notably through a deubiquitination-independent mechanism. Human USP8 is 1118 amino acids (aa) in length. It contains one MIT domain that mediates endosomal trafficking (aa 33-116), a rhodanese domain that binds RNF proteins (aa 184-305), an SH3 domain binding sequence (aa 405-413), and a C-terminal catalytic domain that hydrolyses ubiquitin adducts (aa 776‑1106). USP8 is phosphorylated at Ser702, 705, 784 and 787. There is one oncogenic recombination isoform that fuses the p85 beta -subunit of PI3 kinase to the C‑terminal 363 aa of USP8, beginning with Pro746. Full-length human and mouse USP8 share 82% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol USP8
Entrez
Uniprot