UBE2R2 Antibody

Western Blot: UBE2R2 Antibody [NBP2-15154] - Sample (30 ug of whole cell lysate) A: Jurkat 12% SDS PAGE; antibody diluted at 1:5000.

Western Blot: UBE2R2 Antibody [NBP2-15154] - Sample (50 ug of whole cell lysate) A: mouse liver 12% SDS PAGE; antibody diluted at 1:1000.

UBC3B antibody detects UBC3B protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: UBC3B protein stained by UBC3B antibody (NBP2-15154) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

UBE2R2 Antibody Summary

• Description: Novus Biologicals Rabbit UBE2R2 Antibody (NBP2-15154) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human UBC3B. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBE2R2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for UBE2R2 Antibody

• CDC34B
• CDC34BEC 6.3.2.19
• E2-CDC34B
• FLJ20419
• MGC10481
• UBC3B
• UBC3BUbiquitin-protein ligase R2
• UBE2R2
• Ubiquitin carrier protein R2
• ubiquitin-conjugating enzyme E2 R2
• Ubiquitin-conjugating enzyme E2-CDC34B
• ubiquitin-conjugating enzyme E2R 2
• ubiquitin-conjugating enzyme UBC3B

Background

Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. This gene encodes a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP, enhancing beta-catenin degradation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: UBE2R2
• Uniprot:
  • Q712K3(Human)