UBE2R1/CDC34 Antibody (14B5) - Azide and BSA Free

Western Blot: UbcH3/Cdc34 Antibody (14B5) [NB500-168] - Sample (30 ug of whole cell lysate) A: A549 B: HCT116 10% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: UbcH3/Cdc34 Antibody (14B5) [NB500-168] - Analysis of paraffin-embedded HBL435 xenograft, using CDC34 antibody at 1:200 dilution.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clone: 14B5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

UBE2R1/CDC34 Antibody (14B5) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse UBE2R1/CDC34 Antibody (14B5) - Azide and BSA Free (NB500-168) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full-length human UBE2R1/CDC34 expressed in E. coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDC34
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  293 Whole Cell Lysate
  UBE2R1/CDC34 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for UBE2R1/CDC34 Antibody (14B5) - Azide and BSA Free

• Cdc34
• cell division cycle 34 homolog (S. cerevisiae)
• cell division cycle 34
• E2-CDC34
• EC 6.3.2.19
• UBC3
• UBE2R1
• UBE2R1UBCH3
• ubiquitin carrier protein
• ubiquitin-conjugating enzyme E2 R1
• Ubiquitin-conjugating enzyme E2-32 kDa complementing
• Ubiquitin-conjugating enzyme E2-CDC34
• Ubiquitin-protein ligase R1

Background

Cdc34 is a ubiquitin conjugating enzyme (UBC) or E2 that has been shown to be essential for the G1 to S phase transition in S. cerevisiae. Human Cdc34 fully complements the budding yeast Cdc34 temperature sensitive mutant phenotype. In egg extracts of the frog Xenopus laevis, Cdc34 has been shown to be required for the onset of DNA replication and appears to function in a multi-protein complex. Also, Cdc34 is highly conserved among vertebrates and is likely to function in a similar manner in all eukaryotes to regulate the start of DNA synthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC34
• Entrez:
  • 997(Human)