UBE2F/NCE2 Antibody

Western Blot: UBE2F/NCE2 Antibody [NBP1-44062] - (0.05ug/ml) Analysis of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: UBE2F/NCE2 Antibody [NBP1-44062] - Staining of Human Placenta shows membrane staining in trophoblasts.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

UBE2F/NCE2 Antibody Summary

• Immunogen: Peptide with sequence C-HLRDKEDFRNKVDD corresponding to C-Terminus according to NP_542409.1.
• Epitope: C-Terminus
• Predicted Species: Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: UBE2F
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2.5 - 3.8 ug/ml
  • Immunohistochemistry-Paraffin 2.5 - 3.8 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.05 - 0.2 ug/ml
• Application Notes: WB: Approx. 20 kDa band observed in mouse liver lysates (calculated MW of 21.1 kDa band according to human NP_542409.1 and to mouse NP_080730.1). IHC-P: Human placenta shows membrane staining in trophoblasts-.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for UBE2F/NCE2 Antibody

• EC 6.3.2
• EC 6.3.2.-
• MGC18120
• NCE2
• NCE2Ubiquitin-conjugating enzyme E2 F
• NEDD8 carrier protein UBE2F
• NEDD8 conjugating enzyme
• NEDD8 protein ligase UBE2F
• NEDD8-conjugating enzyme 2
• NEDD8-conjugating enzyme UBE2F
• UBE2F
• ubiquitin-conjugating enzyme E2F (putative)

Background

NCE2 is a ubiquitin conjugating enzyme. Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps for modification include the activation of ubiquitin, an ATP dependent formation of a thioester bond between ubiquitin and the enzyme E1, transfer by transacylation of ubiquitin from E1 to the ubiquitin conjugating enzyme E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse the marking of protein substrates. Posttranslational tagging by Ub is involved in a multitude of cellular processes, including the cell cycle, cell growth and differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, regulation of transcription and DNA replication, transmembrane transport, stress responses, the immune response, and nervous system functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UBE2F/NCE2 Antibody (NBP1-44062)(1)

Publication using NBP1-44062

• Huang DT, Ayrault O, Hunt HW et al. E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. Mol Cell 2009-02-27 [PMID: 19250909]

Bioinformatics

• Gene Symbol: UBE2F
• Entrez:
  • 140739(Human)
  • 67921(Mouse)
  • 363284(Rat)
• Uniprot:
  • NP_542409.1(Human)