![Immunocytochemistry/Immunofluorescence: Ube2L3 Antibody [NBP2-20786] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Ube2L3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20786-img0003.jpg "Immunocytochemistry/Immunofluorescence: Ube2L3 Antibody [NBP2-20786] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.")
| Reactivity | Hu, Mu, Pm, RMSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit UbcH7/UBE2L3 Antibody - BSA Free (NBP2-20786) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human UbcH7/UBE2L3. The exact sequence is proprietary. |
| Predicted Species | Rhesus Macaque (100%), Primate (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | UBE2L3 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 18 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Reviewed Applications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||
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Enlarge |
reviewed by:
Youwei Zhang |
WB | Human | 11/26/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for UbcH7/UBE2L3 Antibody (NBP2-20786)Find related products by research area.
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![Western Blot: Ube2L3 Antibody [NBP2-20786] - Sample (30 ug of whole cell lysate) A: JurKat B: Hela 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Ube2L3-Antibody-Western-Blot-NBP2-20786-img0001.jpg "Western Blot: Ube2L3 Antibody [NBP2-20786] - Sample (30 ug of whole cell lysate) A: JurKat B: Hela 12% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: UbcH7/UBE2L3 Antibody [NBP2-20786] - Whole zebrafish extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with UbcH7/UBE2L3 antibody [N1C3] (NBP2-20786) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-20786_rabbit-polyclonal-ubch7-ube2l3-antibody-910202420122077.jpg "Western Blot: UbcH7/UBE2L3 Antibody [NBP2-20786] - Whole zebrafish extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with UbcH7/UBE2L3 antibody [N1C3] (NBP2-20786) diluted at 1:1000.")
![Western Blot: UbcH7/UBE2L3 Antibody [NBP2-20786] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with UbcH7/UBE2L3 antibody [N1C3] (NBP2-20786) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-20786_rabbit-polyclonal-ubch7-ube2l3-antibody-91020242012208.jpg "Western Blot: UbcH7/UBE2L3 Antibody [NBP2-20786] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with UbcH7/UBE2L3 antibody [N1C3] (NBP2-20786) diluted at 1:1000.")
![Immunohistochemistry: UbcH7/UBE2L3 Antibody [NBP2-20786] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using UbcH7/UBE2L3 antibody [N1C3] (NBP2-20786) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp2-20786_rabbit-polyclonal-ubch7-ube2l3-antibody-91020242015724.jpg "Immunohistochemistry: UbcH7/UBE2L3 Antibody [NBP2-20786] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using UbcH7/UBE2L3 antibody [N1C3] (NBP2-20786) at 1:300 dilution.")
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with UBE2L3 antibody [N1C3] (NBP2-20786) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20786_rabbit-polyclonal-ubch7-ube2l3-antibody-western-blot-2611202518303817.jpg "Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with UBE2L3 antibody [N1C3] (NBP2-20786) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![UbcH5a/UBE2D1 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "UbcH5a/UBE2D1 [Unconjugated]")
![Western Blot Butyrylcholinesterase/BCHE Antibody [Unconjugated]](https://images.novusbio.com/images2/Butyrylcholinesterase_AF9024_Western_Blot_19121.jpg)
![Immunohistochemistry Butyrylcholinesterase/BCHE Antibody [Unconjugated]](https://images.novusbio.com/images2/Butyrylcholinesterase_AF9024_Immunohistochemistry_19158.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
